Introduction: The purpose of this study was to determine Na-Ca exchanger 2, 3 (NCX2, 3) protein level changes during 2, 5, 10, 15 days after induction of normobaric hyperoxia (HO) preconditioning. Materials and Methods: Rats were divided in two experimental groups. The first group was exposed to 95% inspired HO for 4 h/day for 6 consecutive days (HO). The second group acted as control, and was ...

NA /CA2 EXCHANGER (NCX) proteins constitute a family of solute carriers that provide the major pathway for removal of calcium from the cytosol in exchange for three times as many sodium ions. The vertebrate NCX proteins are expressed as tissue-specific subtypes coded by multiple genes (NCX1, NCX2, and NCX3 in mammals) and further diversified by variable splicing (e.g., NCX1.1, NCX1.2, and NCX1....

Ion transport and regulation of Na 1 –Ca 2 1 exchange were examined for two alternatively spliced isoforms of the canine cardiac Na 1 –Ca 2 1 exchanger, NCX1.1, to assess the role(s) of the mutually exclusive A and B exons. The exchangers examined, NCX1.3 and NCX1.4, are commonly referred to as the kidney and brain splice variants and differ only in the expression of the BD or AD exons, respect...

In SH-SY5Y, a human neuroblastoma cell line, Aroclor 1254 (A1254), induced a dose-dependent (10–50 g/ml) intracellular calcium concentration ([Ca ]i) increase. Two rather specific sodium-calcium (Na -Ca ) exchanger (NCX) inhibitors, bepridil (10 M) and KB-R7943 [2-[2-[4-(4-nitrobenzyloxy) phenyl]ethyl]isothiourea methanesulfonate] (10 M), reduced A1254induced [Ca ]i increase. A 24-h exposure to...

Na /Ca exchanger (NCX) is a plasma membrane transporter that moves Ca in or out of the cell, depending on membrane potential and transmembrane ion gradients. NCX is the main pathway for Ca extrusion from excitable cells. NCX inhibitors can ameliorate cardiac ischemia-reperfusion injury and promote high-frequency fatigue of skeletal muscle, purportedly by inhibiting the Ca inward mode of NCX. He...