recombinant protein purification is a kind of sensitive and expensive method in genetics engineering. genetic manipulation leads to the expression of various proteins; it should be isolated with high purity finally. differed methods for protein purification are categorized, based on cast, quality, easy work and side effect of protein. in this article, we are investigating his his-tag protein pu...

two facile and low cost routes were used for the synthesis of if-ws2 nanoparticles. in this paper, preparation of if-ws2 nanoparticles with two different methods was investigated. in the first method, wo3 nanoparticles were reacted with h2s gas in n2/h2 reducing atmosphere at 900 oc. the as-synthesized if-ws2 nanoparticles are spherical shape with an average size of about 70 nm.  in the second ...

we have used lithium aluminum hydroxide reducing method of silver nitrate to prepare heterogeneous silver-silica nanocomposite particles with thiol and amino groups serving to bind the ag nanoparticles to the surfaces of the sio2 nanoparticles. we examined products of these reductions using ftir, sem, xrd and uv-vis. spectroscopy. the sio2 nanoparticles had diameters ranging from 70 to 90 nm; t...

The tolerance of BALB/c mice to different doses of blank and cisplatin-loaded PLGA-mPEG nanoparticles and the in vivo anticancer activity of these nanoparticles on SCID mice xenografted with colorectal adenocarcinoma HT 29 cells were investigated. Nanoparticles with an average size of 150-160 nm and approximately 2% w/w cisplatin content were prepared by a modified emulsification and solvent ev...

The performance of gold and polystyrene nanoparticles was investigated using quartz crystal microbalance (QCM) as sensor platform; β-Galactosidase antibody with corresponding antigen was utilized for the immunoreaction. The development of the immunosensor included: 1) formation of self assembled monolayers on quartz crystals; 2a) immobilization of p-aminothiophenol functionalized gold nanoparti...

Targeting drugs to their sites of action is still a major challenge in pharmaceutical research. In this study, polylactic-co-glycolic acid (PLGA) immuno-nanoparticles were prepared for targeting invasive epithelial breast tumour cells. Monoclonal antibody (mAb) was used as a homing ligand and was attached to the nanoparticle surface either covalently or non-covalently. The presence of mAb on th...

BACKGROUND There is an increasing commercial demand for nanoparticles due to their wide applicability in various markets, including medicine, catalysis, electronics, chemistry, and energy. In this report, a simple and ecofriendly chemical reaction for the synthesis of gold and silver nanoparticles from Trianthema decandra (Aizoaceae) has been developed. METHODS AND RESULTS On treatment of aqu...

1 1 GENERAL INTRODUCTION 3 1.1 Endothelial related diseases 3 1.2 siRNA and the principle of RNA interference 8 1.3 RNA interference in research and therapy 13 1.4 Viral and non-viral gene delivery 14 1.5 Nanoparticles: definitions, history and current state of knowledge 16 1.6 Gelatin based nanoparticles as colloidal drug delivery vectors 18 1.7 Body distribution of nanoparticles 19 1.8 Nanopa...

The antimicrobial properties of silver and copper nanoparticles were investigated using Escherichia coli (four strains), Bacillus subtilis and Staphylococcus aureus (three strains). The average sizes of the silver and copper nanoparticles were 3 nm and 9 nm, respectively, as determined through transmission electron microscopy. Energy-dispersive X-ray spectra of silver and copper nanoparticles r...

Nanoparticles prepared using human serum albumin (HSA) have emerged as versatile carriers for improving the pharmacokinetic profile of drugs. The desolvation of HSA using ethanol followed by stabilization through crosslinking with glutaraldehyde is a common technique for preparing HSA nanoparticles, but our knowledge concerning the characteristics (or functions) of HSA nanoparticles and their e...