Background: The kidneys perform glomerular filtration, tubular reabsorption, and tubular secretion and the study of urinary excretion of some enzymes considered as a sensitive test for the detection of early stages of renal disease, particularly N-acetyl-β-D-glucosaminidase (NAG) which is a hydrolytic lysosomal enzyme present in the epithelial cells of the proximal convoluted tubule. Increased ...

Renal tubular targeted hormones increase urinary excretion of a lysosomal enzyme, N-acetyl-beta-D-glucosaminidase (NAG). To elucidate the mechanism of this event, the calcitonin effect on NAG handling by LLC-PK1 cells was examined. Calcitonin (1 nM to 1 microM), phorbol myristate (10 nM to 1 microM), and ionomycin (1 to 10 microM) promoted NAG release without any increase in lactate dehydrogena...

Calling NAG routines from Visual Basic or Excel can provide a convenient way quickly to develop graphical user interfaces and packages that use mathematical routines The aim of this report is to show how the complete range of NAG C DLL routines can be called directly from Visual Basic and thus allow programmers package builders maximum exibility when incorporating calls to NAG routines into the...

594 CLINICALCHEMISTRY,Vol. 30, No. 4, 1984 that the preservatives used had no effect on the MCP-NAG assay but that NAG activity declined when the urine pH was >8.0 and this decline became more marked on storage. This test kit provides a rapid method of estimating urinary NAG and can be used with a simple spectrophotometer. The lack of any reference standard is a drawback, but we understand it i...

background: all the three nitric oxide synthase (nos) isoforms are presented in the kidney. some data provided evidences that enos activity leads to restoration of renal function after injury, but activation of inos aggravates renal failure. in the present study, we investigated that whether in gentamicin-induced renal failure selective inos blockade is beneficial. materials and methods: four g...

BACKGROUND Diabetic nephropathy is a clinical diagnosis where proteinuria is present in a patient with diabetes. Early intervention can significantly improve the prognosis. However, imprecision of the currently available biomarkers have impaired effective therapies in a timely manner. Urinary N-acetyl-β-D-glucosaminidase (NAG) is excreted in abnormally high amounts in many renal diseases. The a...

PURPOSE To investigate renal function estimated by markers in full-term newborns with hyperbilirubinemia. METHODS A total of 332 full-term newborns with hyperbilirubinemia and 60 healthy full-term newborns were enrolled. Total serum bilirubin, serum creatinine (Cr), serum blood urea nitrogen (BUN), serum cystatin C (Cys-C), urinary beta-2-microglobulin (β 2MG) index, and urinary N-acetyl-beta...