Two skeletal muscle-specific regulatory factors, myogenin and MyoD1, share extensive homology within a myc similarity region and have each been shown to activate the morphologic and molecular events associated with myogenesis after transfection into nonmyogenic cells. The BC3H1 muscle cell line expresses myogenin and other muscle-specific genes, but does not express MyoDl during differentiation...

dear editor:   we thank dr asadi amoli for her interest in our article. 1 dr asadi amoli pointed out that specific and sensitive rhabdomyosarcoma (rms) markers should be investigated in our case. 2 we agree with dr asadi amoli ’s opinion and are sorry that the results of pathology examination and myogenin (myf-4) staining studied in a local hospital were not provided in our case report.   an ex...

Myogenin is a gene belonging to the MyoD family, which codes for the bHLH transcription factor playing a key role in myogenesis. It affects the processes of differentiation and maturation of myotubes during embryogenesis. Fragments of the porcine myogenin coding sequence and promoter region were amplified and subjected to MSSCP analysis. T-->C transition recognised by the MaeIII restriction enz...

We developed a detection system for myogenic regulatory factors such as MyoD and myogenin. Adapting the method we performed a longitudinal analysis of such regulatory factors after infection with T. spiralis and T. pseudospiralis. MyoD and myogenin were expressed from the early phase of cystogenesis in T. spiralis infection. The expression returned to the normal level after 18 days from the inf...

Angiotensin II (Ang II), an important component of the renin–angiotensin system (RAS), plays a critical role in pathogenesis cardiovascular disorders. In addition, human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) have been considered as promising platform for studying personalized medicine heart diseases. However, whether Ang can induce apoptosis hiPSC-CMs is not known. th...

Hormone replacement therapy (HRT) is used in postmenopausal women to relieve symptoms of menopause and prevent osteoporosis. We sought to evaluate changes in mRNA expression of key myogenic factors in postmenopausal women taking and not taking HRT following a high-intensity eccentric resistance exercise. Fourteen postmenopausal women were studied and included 6 control women not using HRT (59 +...

Different mouse muscle cell lines were found to express distinct patterns of myosin heavy chain (MHC) isoforms, MyoD1, and myogenin, but there appeared to be no correlation between the pattern of MHC expression and the patterns of MyoD1 and myogenin expression. Myogenic cell lines were generated from unconverted C3H10T1/2 cells by 5-azacytidine treatment (Aza cell lines) and by stable transfect...

GH and IGF-I control over 80% of postnatal growth. We recently established that TNFalpha impairs the ability of IGF-I to increase protein synthesis and promote expression of myogenin in myoblasts. Here we extend these results by showing that ceramide, a second messenger in both TNFalpha and IL-1beta receptor signaling pathways, is a key downstream sphingosine-based lipid that leads to IGF-I res...

Skeletal muscle differentiation, maturation, and regeneration are regulated by interactions between signaling pathways activated by hormones and growth factors, and intrinsic genetic programs controlled by myogenic transcription factors, including members of the MyoD and MEF2 families. Insulin-like growth factors (IGFs) play key roles in muscle development in the embryo, and in the maintenance ...