Tuberculosis is a vagarious infectious disease that generally affects the lungs. Accordingly, in some cases, it can also affect the liver and kidney. Host genetic may affect tuberculosis caused by bacillus Mycobacterium tuberculosis. The main risk factors for the disease are a weakened immune system because of diabetes, some cancers, HIV/AIDS, severe kidney disease, cancer treatment, a...

Traditional testing methods have limited epidemiologic studies of tuberculosis among free-living primates. PCR amplification of insertion element IS6110 of Mycobacterium tuberculosis from fecal samples was evaluated as a noninvasive screening test for tuberculosis in primates. Active tuberculosis was detected among inoculated macaques and naturally exposed chimpanzees, demonstrating the utility...

objective the aim of this study was to compare the sensitivity and specificity of acid fast and auramine-rhodamine staining and multiplex pcr for the detection of mycobacterium tuberculosiscomplex and non tuberculosis mycobacteria on formalin fixed paraffin embedded tissues (ffpe) materials and methods forty cases of ffpe pleural and bronchial tissue with chronic granulomatous inflammation and ...

This study was aimed to rapidly identify and differentiate two main pathogens of the Mycobacterium tuberculosis complex: Mycobacterium tuberculosis subsp. tuberculosis and Mycobacterium bovis by a modified loop-mediated isothermal amplification (LAMP) assay. The reaction results could be evaluated by naked eye with two optimized closed tube detection methods as follows: ...

conclusions concurrent pulmonary nocardiosis and tuberculosis is frequent in hiv-infected patients. rapid and sensitive methods such as pcr are recommended for detection of such co-infections. results from 157 sputum specimens, positive samples by acid fast staining, culture and pcr for m. tuberculosis were reported for 7.6% (12/157), 10.1% (16/157) and 7% (11/157) of samples, respectively. no ...

The detection of Mycobacterium tuberculosis by culture of cerebrospinal fluid (CSF) is unacceptably slow. Low numbers of organisms and the presence of reaction inhibitors may prevent detection of M. tuberculosis by PCR. We used immunomagnetic enrichment to accelerate and enhance the detection of mycobacteria in CSF after demonstrating the utility of the method with pure suspensions. Growth was ...

Dear Editor-in-Chief We read with interest the study by Khazaei et al. (1) in which the authors have nicely concluded that PCR is more sensitive test than Ziehl-Neelsen staining and histo-pathological examination for the diagnosis of tuberculosis (TB). They have rightly pointed to use PCR, selectively, in acidfast bacilli negative paucibacillary forms of TB. However, we intend to highlight few...

Abbreviations used: DMSO, Dimethyl sulfoxide; E. coli, Escherichia coli; FP, 15 Fluorescence Polarization; LB, Luria-Bertani; MDR-TB, Multi-Drug Resistant 16 Tuberculosis; MALDI, Matrix-Assisted Laser Desorption/Ionization; MIC, 17 Minimum Inhibitory Concentration; M. smegmatis, Mycobacterium smegmatis; 18 Mtb, Mycobacterium tuberculosis; MTT, (3-(4,5-Dimethylthiazol-2-yl)-2,519 diphenyltetrazo...