Background:Pseudomonas aeruginosa is a gram negative non facultative bacterium and one of the members of normal flora in different sites of body in healthy humans.this bacterium can resist in fluids and hospital environments for a long time.Pseudomonas aeruginosa has two systems for glucose uptake:a low affinity oxidative pathway and a high affinity phosohorylative pathway.Although the role of ...

Thermostable chitinases are useful for industrial and biotechnological applications. This paper reports the stabilization of chitinase from Serratia marcescens B4A through rational mutagenesis. Changing of Ser 390 to Ile in S. marcescens. The stabilization was enhanced through entropic stabilization by reduction of the loop length and also by increasing of the beta chain length. With this repla...

There is no functional annotation for the majority of the several hundreds of receptor-like kinases in plants. A direct way of inferring the function of these proteins is to study the phenotype that results from loss of function mutants such as T-DNA mutant lines. In this research a function (phenotype) to At2g37050 gene that encodes a receptor like kinase in Arabidopsis T-DNA line was...

Three rat sarcoma (RAS) gene isoforms, KRAS, NRAS, and HRAS, constitute the most mutated family of small GTPases in cancer. While development targeted immunotherapies has led to a substantial improvement overall survival patients with non-KRAS-mutant cancer, RAS-mutant cancers have an poorer prognosis owing high aggressiveness tumors. KRAS mutations are strongly implicated lung, pancreatic, col...

in order to induce of genetic variation and selection of genotypes with desirable traits, seeds of three varieties of rapeseed sarigol (pf), rgs003 and zarfam were treated with different doses of gamma ray 500, 700 and 900 gy. to confirm the stability of the induced genetic traits, 66 mutant lines from m4 generation were studied in a randomized complete block design with three replications. twe...

isoniazid, is the only antituberculous drug for which the relation between lack of virulence and acquisition of resistance was associated. inh-resistant mutants were shown to contain defective katg gene. classical studies showed that inh-resistant south indian isolates have lower virulence in guinea pigs and higher susceptibility to h2o2. it is of interest to assess the virulence in south india...

objective(s):[p1]  this study highlights xylanase overproduction from bacillus mojavensis via uv mutagenesis and optimization of the production process. materials and methods:bacillus mojavenis ptcc 1723 underwent uv radiation. mutants’ primary screening was based on the enhanced hollow zone diameter/ colony diameter ration (h/c ratios) of the colonies in comparison with the wild strain on xyla...