نتایج جستجو برای: multiple structural breaksjel classification o1
تعداد نتایج: 1575707 فیلتر نتایج به سال:
Bacteriophage Felix O1, also known as enterobacteria phage O1, has been used to type Salmonella Typhi and is an excellent candidate for use in bioremedial and therapeuticapplications. It has extremely high intra-species specificity and is strictly virulent in nature,unable to undergo lysogeny. To facilitate the development of the bacteriophage for use in theseareas, the full sequenc...
The azoospermia factor c (AZFc) region in the long arm of human Y chromosome is characterized by massive palindromes. It harbors eight multi-copy gene families that are expressed exclusively or predominantly in testis. To assess systematically the role of the AZFc region and these eight gene families in spermatogenesis, we conducted a comprehensive molecular analysis (including Y chromosome hap...
A novel, sensitive locus-specific touchdown-multiplex polymerase chain reaction (TMPCR), which is based on two-stage amplification pertaining to multiplex PCR and conditional touchdown strategy, was used in detecting and differentiating Vibrio cholerae serogroups. A panel of molecular marker-based TMPCR method generates reproducible profiles of V. cholerae-specific (588 bp) amplicons derived fr...
BACKGROUND Vibrio cholerae O1 El Tor dominated the seventh cholera pandemic which occurred in the 1960s. For two decades, variants of V. cholerae O1 El Tor that produce classical cholera toxin have emerged and spread globally, replacing the prototypic El Tor biotype. This study aims to characterize V. cholerae O1 isolates from outbreaks in Thailand with special reference to genotypic variations...
Genes located on the CTX element and the Vibrio cholerae pathogenicity island (VPI) were investigated in 297 clinical V. cholerae O1 and 76 environmental O1 and non-O1 isolates from Brazil between 1991 and 1999. RAPD analysis suggested that serogroup O1 strains regardless of clinical or environmental source were clonal while non-O1 strains showed greater diversity. PCR analysis showed that 71% ...
Toxigenic Vibrio cholerae strains are lysogens of CTXF, a filamentous phage which encodes cholera toxin. The receptor for CTXF for invading V. cholerae cells is the toxin-coregulated pilus (TCP), the genes for which reside in a larger genetic element, the TCP pathogenicity island. We analyzed 146 CTX-negative strains of V. cholerae O1 or non-O1 isolated from patients or surface waters in five d...
Vibrio cholerae, a member of the family Vibrionaceae, is a facultatively anaerobic, Gram-negative, non-spore-forming curved rod, about 1.4–2.6 mm long, capable of respiratory and fermentative metabolism; it is well defined on the basis of biochemical tests and DNA homology studies (Baumann, Furniss & Lee, 1984). The bacterium is oxidase-positive, reduces nitrate, and is motile by means of a sin...
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