Inter-domain communication plays a key role in the function of modular proteins. Earlier studies have demonstrated that the coupling of domain motions is important in mediating site-to-site communications in modular proteins. In the present study, bioinformatics and molecular simulations were used to trace “pre-existing” residue-residue interaction networks that mediate coupled-domain dynamics ...

Background: Analysis of pesticide residues in food and other environmental commodities have become an essential requirement for consumers, producers, food inspectors and authorities. This study is focused on validation of an accurate, rapid and reliable method for multi-residual analysis of pesticides in pistachio as a strategic crop for export and one of the main nuts in Iranian food basket. M...

objective: the usage of pesticides has increased during the recent years. one the other hand, presence of harmful pesticides residue in rice and other food has caused a great concern among the consumers. therefore, it is important to develop some pragmatic procedures to increase the food safety along with the reduction in consumption of pesticide residues. methods: a gas chromatograph with elec...

in this study the effect of different nitrogen source substrates on the amount of chitosan production by aspergillus niger was investigated. a. niger ptcc 5012 from the persian type culture collection (ptcc) was grown on soy bean, corn seed and canola residues at 30 °c for specified cultivation days under sterilized conditions. chitosan was extracted from the fungal mycelia using hot alkaline a...

Criteria are presented by which analytical methods may be judged to have been validated for the determination of pesticide residues. All stages of analysis are addressed, from initial preparation of samples to the production of results, but with a focus on simplicity and cost-effectiveness of the requirements. Criteria are provided for both quantitative and qualitative (screening) methods and t...

Rheumatoid Arthritis (RA) is a prevalent autoimmune and inflammatory disease that requires restructuring. A lot of research information available, but clear etiology drug target still unclear. bottom-up approach can add more to existing knowledge about RA. One better way understanding the disease-related mechanism objectives be detailed residue-residue interaction proteins involved with In curr...

A multi-residue method based on modified QuEChERS sample preparation with multi-walled carbon nanotubes (MWCNTs) as reversed-dispersive solid phase extraction (r-DSPE) material and gas chromatography-mass spectrometry determination by selected ion monitoring (GC/MS-SIM) mode was validated on 30 representative pesticides residues in vegetables and fruits. The acetonitrile-based QuEChERS (quick, ...

A multi-residue method for the determination of 54 pesticide residues in pollens has been developed and validated. The proposed method was applied to the analysis of 48 crude pollen samples collected from eight provinces of China. The recovery of analytes ranged from 60% to 136% with relative standard deviations (RSDs) below 30%. Of the 54 targeted compounds, 19 pesticides were detected. The ma...

Rapid multi-residue analysis of pesticides in agricultural products was studied by using LC-MS/MS. Pesticide residues in 10 g of homogenized agricultural products were extracted with 30 mL of acetonitrile and salted out with 4 g of anhydrous magnesium sulfate and 1 g of sodium chloride in the presence of citrate salts for buffering in a disposable tube. Co-extractives were removed by use of our...