Here, efficient plantlet regeneration via somatic embryogenesis from Panax quinquefolium hairy roots was reported. Callus development was optimal on MS medium containing 2,4-dichlorophenoxyacetic acid (1 mg l) and -naphthalene acetic acid (1 mg l). After a 4-week growth, calli were transferred onto MS medium with different combinations of plant growth regulators (PGRs). The highest embryogenesi...

Punicagranatum cv. Malassaveh is one of the most desirable commercial cultivars in Iran. In thisstudy some experiments were conducted to introduce the best hormonal treatments for directadventitious shoot regeneration from mentioned cultivar via cotyledonary explants. For thispurpose, both liquid and agarMurashige and Skoog (1962) (MS) medium supplemented with BAat 2, 4.5, 9, 13 μM alone and in...

In vitro propagation of AZ x M9 new apple rootstock resulted of breeding program of vegetative apple rootstock in Iran was investigated. Nodal explants were inoculated in Murashige and SKoog (MS) medium supplemented with various concentrations of 6-benzylaminon purine (BAP) (0.4, 0.8 and 1.2 mg/l) alone or with naphthalene acetic acid (NAA) (0.1 mg/l). We examined the effect of differe...

An efficient protocol for in vitro shoot multiplication of Randia dumetorum (Emetic nut) has been developed. The seeds of R. dumetorum were germinated in vitro in MS medium in 5 weeks. Subsequent propagation using shoot tip as an explant was carried out in MS medium along with different concentrations and combinations of BAP (0.5-2.0) and NAA (0.0-2.0). Maximum shoot multiplication was obtained...

Tissue culture methods for plant regeneration have been developed for Lasiurus scindicus, Sorghum halepense and Urochloa panicoides using basal portion of young inflorescence as new and a potential source(s) of explants. Highly embryogenic cultures were initiated on Murashige and Skoog’s (MS) medium supplemented with various concentrations of 2,4-dichlorophenoxyacetic acid (2-4,D). The regenera...

In vitro regeneration of plants from root cultures of Swertia chirata Buch.-Ham. ex Wall was obtained. Root explants from axenic shoot cultures were used for shoot induction. Optimal shoot regeneration without any callus intervention was observed on 1/2 MS (Murashige and Skoog’s medium, 1962) + 4.44 μM 6, benzylaminopurine (BAP) +1.07 μM α-naphthalene acetic acid (NAA).Regenerated shoots were f...

Licorice plants, Glycyrrhiza glabra, G. uralensis, and G. inflata, were investigated for callus induction using Murashige and Skoog (MS) medium combined with auxins and cytokinins. After 4 weeks of culture, 33-100% of leaf or stem explants formed calli. Maximum of shoot induction from callus cultures was achieved by G. inflata stem explants cultured on MS medium supplemented with 1 mg/l alpha-n...

An efficient in vitro plant regeneration protocol was developed for Chrysanthemum morifolium, an ornamental plant. Nodal segments were used as explants in the present experiment. The explants were cultured onto Murashige & Skoog’s (MS) nutrient medium containing plant growth regulators (cytokinins or auxins) with various combinations and concentrations for the study. Maximum percentage (93.33%)...

Shoot tip and nodal segment explants of Holarrhena antidysenterica when cultured on MS medium containing BAP (1.0-3.0 mg/l) with NAA (0.2-1.0 mg/l) and BAP (1.0-3.0 mg/l) with Kn. (0.2-1.0 mg/l) produced multiple shoots. Maximum multiple shoots was found in MS medium supplemented with BAP (2.0 mg/l) and NAA (0.5 mg/l). Subculture on the same medium resulted in rapid shoot multiplication at an a...