Two new oxidovanadium(V) complexes, (HNEt3)[VVO2L] (1) and [(VVOL)2?-O] (2), have been synthesized using a tridentate Schiff base ligand H2L [where = 4-((E)-(2-hydroxy-5-nitrophenylimino)methyl)benzene-1,3-diol] VO(acac)2 as starting metal precursor. The corresponding complexes are characterized by physicochemical (elemental analysis), spectroscopic (FT-IR, UV–Vis, NMR), spectrometric (ESI–MS) ...

Balanced deoxyribonucleotides pools are essential for cell survival and genome stability. Ribonucleotide reductase is the rate-limiting enzyme for the production of deoxyribonucleotides. We report here that p53 suppresses ribonucleotide reductase subunit 1 (RRM1) and 2 (RRM2) via inhibiting mammalian target of rapamycin complex 1 (mTORC1). In vitro, cancer cell lines and mouse embryonic fibrobl...

This paper describes a method for quantitative extraction of nucleic acids from cultured pre-implantation mouse embryos in a form suitable for electrophoresis, and its application to the analysis of RNA synthesized during early mouse development. The nucleic acids synthesized by early mouse embryos have been identified by the use of RNase, DNase and mild alkaline hydrolysis. No obvious differen...

Large-scale gene expression measurements with oligonucleotide microarrays have contributed tremendously to biological research. However, to distinguish between relevant expression changes and falsely identified positives, the source and magnitude of errors must be understood. Here, we report a source of biological variability in microarray experiments with stably transfected cell lines. Mouse e...

background:   objective: this study presents an economical and simple co-culture system for pluripotent stem cells generation from neonatal mouse testis   materials and methods: isolated testicular cells were cultured in dmem/f12. characteristics of the isolated cells and obtained es-like cell were immune-cytochemically confirmed by examining the presence of plzf, vimentin, oct4 and nanog prote...

Background: The main goals of our study were as follows: (1) to investigate whether the methanolic extract of the new cultivar (Gürarslan) of Trigonella foenum graecum L. seeds exhibit antiproliferative effects on the HeLa human cervix carcinoma, Mat-LyLu rat prostate carcinoma and 3T3 embryonic mouse fibroblast cell lines, (2) to determine how mitosis and DNA synthesis are affected in cancer c...

How do we study the endocrine cell differentiation of the placenta? The accepted course of action dictates that you use primary trophoblast cell cultures derived from human placentas. This is accomplished using methods to isolate cytotrophoblasts that possess the potential to spontaneously differentiate into endocrinologically active syncytial trophoblast within a prescribed period of time (1)....

Human embryonic stem cells (hESCs) are routinely cultured on fibroblast feeder layers or in fibroblast-conditioned medium (CM). Bone morphogenetic proteins (BMPs) have previously been shown to induce hESC differentiation, in apparent contrast to mouse embryonic stem (ES) cells, in which BMP4 synergizes with leukemia inhibitory factor (LIF) to maintain self-renewal. Here we demonstrate that hESC...

The design of improved biopolymeric based hydrogel materials with high load-capacity to serve as biocompatible drug carriers is a challenging task vital implications in health sciences. In this work, chitosan crosslinked dialdehyde xanthan gum interpenetrated hydroxypropyl methylcellulose gels were developed for the controlled delivery different antibiotic drugs including ampicillin, minocyclin...

A 6.7-kilobase met complementary DNA (cDNA) was isolated from a pcD cDNA library prepared from C3H mouse fibroblast cell line polyadenylated RNA. Sequence analysis of 6.7-kilobase met cDNA insert revealed that it contained the entire open reading frame and shared an overall homology of 88.1% with the human met gene. Using the mouse met cDNA as probe, high levels of met expression were observed ...