Cytosolic 5′-nucleotidase II (cN-II) and ecto-5′-nucleotidase (CD73) are enzymes involved in the nucleotide metabolism by dephosphorylating nucleoside monophosphates. Both cancer modifying anticancer drug activity, cell biology immune modulation. We have modified lung cells (NCI-H292) to become deficient for either or both using CRISPR/Cas9 technique, studied implication of two cellular respons...

Apyrases are a recurrent feature of secretomes from numerous species parasitic nematodes. Here we characterise the five apyrases secreted by Heligmosomoides polygyrus, natural parasite mice and widely used laboratory model for intestinal nematode infection. All enzymes closely related to soluble calcium-activated nucleotidases described in variety organisms, distinct CD39 family ecto-nucleotida...

We herein describe the first synthesis of iminosugar C-glycosides of α-D-GlcNAc-1-phosphate in 10 steps starting from unprotected D-GlcNAc. A diastereoselective intramolecular iodoamination-cyclization as the key step was employed to construct the central piperidine ring of the iminosugar and the C-glycosidic structure of α-D-GlcNAc. Finally, the iminosugar phosphonate and its elongated phospha...

The gene dnph1 (or rcl) encodes a hydrolase that cleaves the 2'-deoxyribonucleoside 5'-monophosphate (dNMP) N-glycosidic bond to yield a free nucleobase and 2-deoxyribose 5-phosphate. Recently, the crystal structure of rat DNPH1, a potential target for anti-cancer therapies, suggested that various analogs of AMP may inhibit this enzyme. From this result, we asked whether N (6)-substituted AMPs,...

An enzyme, 5’-hydroxyl polyribonucleotide kinase, which catalyzes the phosphorylation of 5’-hydroxyl ends of RNA in the presence of ATP, has been isolated from extracts of HeLa cell nuclei. The kinase requires a divalent cation (Mg’+ or Mn’+) for activity, has an alkaline pH optimum, and is sensitive to the sulfhydryl antagonist N-ethylmaleimide. 5’-Hydroxyl terminated polydeoxyribonucleotides ...

An enzyme, 5'-hydroxyl polyribonucleotide kinase, which catalyzes the phosphorylation of 5'-hydroxyl ends of RNA in the presence of ATP, has been isolated from extracts of HeLa cell nuclei. The kinase requires a divalent cation (Mg2+ or Mn2+) for activity, has an alkaline pH optimum, and is sensitive to the sulfhydryl antagonist N-ethylmaleimide. 5'-hydroxyl terminated polydeoxyribonucleotides ...