1. A number of reactive triazine dyes specifically and irreversibly inactive yeast hexokinase at pH 8.5 and 33 degrees C. Under these conditions, the enzyme is readily inactivated by 100 microM-Procion Green H-4G, Blue H-B, Turquoise H-7G and Turquoise H-A, is less readily inactivated by Procion Brown H-2G. Green HE-4BD, Red HE-3B and Yellow H-5G and is not inactivated at all by Procion Yellow ...

The two-dimensional monomer-monomer (AB) surface reaction model without diffusion is considered for infinitesimal, finite, and infinite reaction rates k. For equal reactant adsorption rates, in all cases, simulations reveal the same form of slow poisoning, associated with clustering of reactants. This behavior is also the same as that found in simulations of the two-dimensional voter model stud...

There is great interest in using microfluidic channels packed with a stationary phase for chemical separations of complex mixtures. A key advantage of such techniques is the use of electroosmotic flow (EOF), controlled simply by applying electrical potentials between reservoirs. A disadvantage for this technique, however, is a lack of compatibility with gradient elution separations. This limita...

In this study, ultrasonic-assisted matrix solid-phase dispersion (UA-MSPD) as a new sample preparation method for natural product analysis was developed. Influential parameters of the UA-MSPD method was optimized using oleuropein content of olive leaves as a model analyte. Main parameters of the proposed technique such as ultrasonic time, amplitude and pulse, sorbent material, the ratio of samp...

Acrylic base resins are widely used in orthopedics and dental surgery. It is generally accepted that due to the incomplete conversion of methyl methacrylate (MMA) monomer to the polymer form during polymerization of the resin, some MMA monomers remain in the hardened material. MMA monomer has been reported to cause abnormalities or lesions in several organs of animals. Study of the literatures ...

Drug elution properties of TiO(2) nanotube arrays have been largely investigated by means of solely macroscopic observations. Controversial elution performances have been reported so far and a clear comprehension of these phenomena is still missing as a consequence of a lack of molecular investigation methods. Here we propose a way to discern drug elution properties of nanotubes through the eva...

Two-stage revision surgery for infected total knee replacement offers the highest rate of success for the elimination of infection. The use of articulating antibiotic-laden cement spacers during the first stage to eradicate infection also allows protection of the soft tissues against excessive scarring and stiffness. We have investigated the effect of cyclical loading of cement spacers on the e...