DNA double-strand breaks (DSBs) are mostly repaired by nonhomologous end joining (NHEJ) and homologous recombination (HR) in higher eukaryotes. In contrast, HR-mediated DSB repair is the major double-strand break repair pathway in lower order organisms such as bacteria and yeast. Penaeus monodon, commonly known as black tiger shrimp, is one of the economically important crustaceans facing large...

The application of effective probiotics in shrimp aquaculture is an excellent alternative for chemicals and antibiotics to prevent disease control. This study was carried out to evaluate the probiotic potential of Lactobacillus acidophilus 04 (home made curd isolate) on pathogenic Vibrio in shrimp Penaeus monodon juveniles. L. acidophilus showed antibacterial activity against Vibrio parahaemoly...

A study was undertaken to determine the most effective element among the commonly used antimicrobial agents, especially in the body muscle of black tiger shrimp (Penaeus monodon) and giant freshwater prawn (Macrobrachium rosenbergii) on the basis of different chemical interventions. Using 10 ppm calcium hypochlorite, the microbial load has been reduced more significantly (p>0.001) than trisodiu...

This study presents updated information on six exotic aquatic species (five crustaceans and one fish): Macrobrachium rosenbergii, Penaeus monodon, Charybdis hellerii, Balanus trigonus, Amphibalanus improvisus, Pterois volitans. The research is based bibliographic available in scientific articles published by the Research Group Fishing Evaluation of Tropical Fishery Resources Biological Photogra...

A new rapid method for titrating baculovirus stocks has been developed using a novel cell line Sf-9 Easy Titer (Sf-9ET). The Sf-9ET cell line has been transfected with plasmid DNA containing the enhanced green fluorescent protein (eGFP) gene under the control of the baculovirus polyhedrin promoter. When used in the titration assay, the Sf-9ET cells turn green when they are infected with baculov...

UNLABELLED A large double-stranded DNA (dsDNA) virus that produces occlusion bodies, typical of baculoviruses, has been described to infect crane fly larvae of the genus Tipula (Diptera, Tipulidae). Because of a lack of genomic data, this virus has remained unclassified. Electron microscopy of an archival virus isolated from Tipula oleracea, T. oleracea nudivirus (ToNV), showed irregularly shap...

Gene therapy in the liver requires powerful vectors capable of mediating sufficient gene delivery and expression in affected hepatocytes. Viral vectors are amongst the most efficient tools for gene delivery, and the search for tissue-specific infecting viruses is important for the development of in v i v o gene therapy strategies. We have recently shown for the first time that a genetically mod...

Baculoviruses are commonly used for recombinant protein and vaccine production. Baculoviruses are nonpathogenic to vertebrates, have a large packaging capacity, display broad host and cell type tropism, infect both dividing and nondividing cells, and do not elicit strong immune or allergic responses in vivo. Hence, their use as gene delivery vehicles has become increasingly popular in recent ye...

Baculoviruses are DNA viruses that can naturally control insects and form the basis for a range of biological insecticides. These occluded in crystalline protein matrix, occlusion body (OB), which favors their persistence environment until consumed by susceptible insect. This review presents evidence baculoviruses perfectly adapted to persist soil. In support this assertion, detailed is present...

Two forms of the squat lobster Pleuroncodes monodon can be found along the Pacific coast of South America: a smaller pelagic and a larger benthic form that live respectively in the northern and southern areas of the geographic distribution of the species. The morphological and life history differences between the pelagic and benthic forms could be explained either by genetic differentiation or ...