The thesis describes the synthesis of chromatographic media using several different approaches, their characterizations and applications in liquid chromatography. The steps to achieve a separation column for a specific analyte are presented. The main focus of the study was the design of novel molecularly imprinted polymers. Attachment of monolithic polymeric substrates to the walls of fused sil...

A new interfacial nano and molecular imprinting approach is developed to prepare spherical molecularly imprinted polymers with well-controlled hierarchical structures. This method is based on Pickering emulsion polymerization using template-modified colloidal particles. The interfacial imprinting is carried out in particle-stabilized oil-in-water emulsions, where the molecular template is prese...

Superparamagnetic nanoparticles are of great current interest for biomedical applications in both diagnostics and treatment. Magnetic nanoparticles (MNP) can be manipulated by magnetic fields, so that when functionalized, they can be used for the purification and separation of biomolecules and even whole cells. Here we report combining the separation capabilities of MNPs with the functional (bi...

The system of polyacrylamide incorporated with methacrylic acid and 2-(dimethylamino)ethyl methacrylate was studied for the possibility of imprinting of lysozyme. The results show that approximately 27% (w/w) of the lysozyme template was not able to be extracted from the molecularly imprinted acrylamide polymers. The amount of the lysozyme template able to be extracted was increased by the addi...

An important challenge for scientific research is the production of artificial systems able to mimic the recognition mechanisms occurring at the molecular level in living systems. A valid contribution in this direction resulted from the development of molecular imprinting. By means of this technology, selective molecular recognition sites are introduced in a polymer, thus conferring it bio-mime...

Molecularly imprinted polymers are versatile materials with wide application scope for the detection, capture and separation of specific compounds present in complex feed stocks. A major challenge associated with their preparation has been the need to sacrifice one mole equivalent of the template molecule to generate the complementary polymer cavities that selectively bind the target molecule. ...

Chlorpyrifos belongs to an organophosphate pesticides group that is frequently used increase the yield of crops by controlling plant and pests-related diseases. contaminates environment cause diseases human population. Molecularly imprinting technology lead development molecularly imprinted polymers having templated oriented cavities with high selectivity, sensitivity, stability, portability. O...

Molecularly imprinted polymers (MIPs) represent a new class of materials possessing high selectivity and affinity for the target molecule. They have been utilized as sensors, catalysts, sorbents for solid-phase extraction, stationary phase for liquid chromatography, mimics of enzymes, receptors, and antibodies. In this research, molecular imprinted polymers (MIPs) for luteolin were prepared usi...