Recombinant protein production in Pichia pastoris is based on alcohol oxidase promoterswhich are regulated by methanol. However, the use of methanol has several disadvantages,which is why current trends in bioprocess development with Pichia pastoris (P. pastoris) arefocusing on methanol mixed feeding strategies. This work aimed to develop a new experimentalmethod and compare the effect of vario...

Microbial growth on mixtures of substrates is of considerable engineering and biological interest. Most of the work until now has dealt with microbial growth on binary mixtures of sugars or polyols. In these cases, it is often found that no matter how the inoculum is precultured, only one of the two substrates is consumed in the first growth phase, leading to the diauxic growth pattern. The goa...

different levels of grape pomace extract (ge) were evaluated for their effect on in vitro gas production and as well on some such ruminal fermentation parameters as apparent degraded substrate, volatile fatty acids (vfa), and microbial protein production, in sheep, as ruminal inoculum donor. as for extraction, dried grape pomace and distilled water were mixed in an equal weight ratio. ge was ad...

Methanotrophs are unique and ubiquitous bacteria that utilize methane as a sole source of carbon and energy from the atmosphere. Besides, methanotrophs may also be targeted for bioremediation of diverse type of heavy metals and organic pollutants owing to the presence of broad-spectrum methane monooxygenases enzyme. They are highly specialized group of aerobic bacteria and have a unique capacit...

The role of TPNH as the donor of reducing equivalents to microsomal mixed function oxidations of drugs, other foreign compounds, and steroids in the liver is well established (1-5). DPNH will not substitute for TPNH in the cyt#{243}chrome P-450-mediated oxidation of drugs, but reaction rates are increased when both nucleotides are present (61 1 ). Although this synergistic role of DPNH has been...