introduction: recent studies have shown the presence of cl- channels in heart and liver mitochondrial membranes. in this work, we have characterized the functional profile of a cl- channel from rat brain mitochondria. methods: after removing and homogenizing the rat brain, the supernatant was separately centrifuged in msedigitonin, h2o and na2co3 and mitochondrial inner membrane vesicles were o...

objective(s): investigation of acute effect on cellular bioenergetics provides the opportunity to characterize the possible adverse effects of drugs more comprehensively. this study aimed to investigate the changes in biochemical and biophysical properties of heart mitochondria induced by captopril and nifedipine antihypertensive treatment. materials and methods: male, 12-week-old wistar rats i...

objective(s) varicocele is associated with impaired testicular function and male infertility, but the molecular mechanisms by which fertility is affected have not been satisfactorily explained. the aim of our study was to investigate whether or not the polymerase gamma (polg) polymorphism is associated with iranian varicocele patients.    materials and methods we determined the polg cag repeat ...

conclusions our results suggest that h. parva and h. oculata may be promising therapeutic candidates for the treatment of hcc, following further confirmatory in vivo experiments and clinical trials. results our results showed that h. parva methanolic extracts (250, 500, and 1000 µg/ml) and h. oculata methanolic extracts (200, 400, and 800 µg/ml) increased reactive oxygen species (ros) formation...

multiple sclerosis (ms) is a neurodegenerative and autoimmune disease that it’s molecular etiology and factors involving in its progression remains unknown. in this study for evaluation effect of mercuric on progression of ms we investigated the additive effect of mercuric sulfide on the brain mitochondrial dysfunction in experimental autoimmune encephalomyelitis (eae) model of ms in c57bl/6 mi...

in this research, we investigated the cytotoxic mechanisms of cochlodinium polykrikoidescell lysate on isolated rat liver hepatocytes.this micro algae is responsible for a severe and widespread harmful algal bloom in the persian gulf and gulf of oman (2008-2009). isolated hepatocytes were obtained by collagenase perfusion of sprague-dawley rat liver.according to our results, incubation of algal...

6- mercaptopurine (6-mp) is widely used in clinic as an immunosuppressive for treatment of acute lymphocytic leukemia, crohn's disease, and ulcerative colitis with documented unpredictable hepatotoxicity. the potential molecular cytotoxic mechanisms of 6-mp against isolated rat hepatocytes were searched in this study using “accelerated cytotoxicity mechanism screening (acms)” techniques. t...

the activity of aspartate aminotransferase (ast) in human serum has been widely determined as a diagnostic aid in liver disease. in this study, the effect of aluminium on ast isoenzymes in relation to aluminium intoxified patients has been investigated. using gel filtration chromatography technique with sephacryl s-300, mitochondrial aminotransferase (m-ast) and cytosolic aminotransferase (c-as...

the radiation-induced bystander effect is the phenomenon which non-irradiated cells exhibit effects along with their different levels as a result of signals received from nearby irradiated cells. responses of non-irradiated cells may include changes in process of translation, gene expression, cell proliferation, apoptosis and cells death. these changes are confirmed by results of some in-vivo s...

mitochondria play an important role in energy production for the cell. the proper function of a myocardial cell largely depends on the functional capacity of the mitochondria.  therefore it is necessary to establish a novel and reliable method for a non-invasive assessment of mitochondrial function and metabolism in humans. although originally designed for evaluating myocardial perfusion, 99mtc...