As a new freshwater aquaculture product, triploid loaches (Misgurnus anguillicaudatus) are characterized by fast growth, high-quality meat, high edibility, resistance to disease, and sterility. In this study, natural tetraploid loach (4n = 100) (?) was crossed with diploid (2n 50) (?), thus creating the hybrid (3n 75). The histological observations of offspring controls at 4 days post-hatching ...