OBJECTIVES We evaluated the changes in antibiotic resistance from 1998 to 2009 of Klebsiella pneumoniae isolated from the intensive care units (ICUs) and urology services of 14 Dutch hospitals and the consequences for empirical therapy. METHODS Quantitative antibiotic susceptibility testing of K. pneumoniae was performed in a central laboratory using a microbroth dilution method. Breakpoints ...

Listeria monocytogenes is one of the major causes of infections in developing countries. In this study, chemical composition and anti-listerial effect of the essential oil of Zataria multiflora Boiss. alone and in combination with monolaurin were evaluated at different pH values (5, 6, and 7) and temperatures (5 ˚C and 30 ˚C). Chemical composition of Zataria multiflora Boiss. essential oil was ...

OBJECTIVES The aim of the study was to analyse the susceptibility of unique and non-duplicate aerobic and anaerobic isolates from surgical patients to a novel des-F(6)-quinolone (garenoxacin) and other selected antimicrobial agents. METHODS Eleven hundred and eighty-five aerobic and anaerobic isolates from general, vascular, cardiothoracic and otolaryngologic surgical patients were tested for...

Background: Calf diarrhea is an important disease that is caused by different pathogens including bacteria, virus and parasites and is associated with economic losses. Objectives: In this study, we evaluated the antibacterial activities of Zatacin (Z. multiflora aerial part ethanol extract), colistin, co-Trimoxazole and enrofloxacin against clinical isolates of Salmonella sp, E. coli and Campyl...

A rapid and high yield microwave assisted synthesis of a series novel 7,9-bis-(arylidene)-4-methyl-2,6,10-triphenyl-2,3-diazaspiro[4,5]dec-3- -ene-1,8-dione has been explored. The spiro diarylidene derivatives having nitrogen atom ?,?-unsaturated ketone moiety were synthesized by aldol condensation between 4-methyl-2,6,10-triphenyl-2,3-diazaspiro[4,5]dec-3-ene-1,8- -dione corresponding aryl ald...

The phenomenon of antimicrobial resistance threatens our ability to treat common infections. clinical pipeline for new antimicrobials is pretty much dry and hence, there a need the development agents with low toxicities help fight resistant microorganisms. This work aimed design peptides using database filtering technology evaluate their bioactivities. physicochemical properties designed were e...

When antibiotic sensitivity of microorganisms is tested by agar dilution, single antibiotics are incorporated in solid media and 'spot' inocula of test strains are applied to the antibiotic media and to a plate without antibiotic. The usual method of quality control is to include control strains of known sensitivity.1 However, control strains are usually sensitive to a number of antibiotics, an...

Sir, In a recent issue of this Journal, Verhaegen & Verbist reported on the in-vitro activities of 21 -lactams against penicillin-susceptible and -resistant isolates of Strepto coccus pneumoniae. The results of this investigation are surprising because they suggest that amoxycillin is less active than penicillin against these strains. We have recently determined the in-vitro susceptibilities of...

Synergistic combinations of antimicrobial agents with different mechanisms of action have been introduced as more successful strategies to combat infections involving multidrug resistant (MDR) bacteria. In this study, we investigated synergistic antimicrobial activity of Camellia sinensis and Juglans regia which are commonly used plants with different antimicrobial agents. Antimicrobial suscept...

The essential oil produced from the seed of Anethum graveolens L. (Umbelliferae) was tested in vitro and in vivo anti-Candida activity. The microbroth dilution method was used in the minimal inhibitory concentration (MIC), according to M27-A3 of the guidelines of the Clinical and Laboratory Standard Institute (CLSI). And then, efficacy evaluation of essential oil in the prophylaxis and treatmen...