INTRODUCTION Postoperative adhesions after abdominal or pelvic surgery remain an important clinical problem causing infertility, pain and bowel obstruction. Their prevention and treatment remains poorly understood and inadequate. The formation of adhesions is caused by the organization of a fibrin matrix, an organization that takes place during the coagulation process when there is suppression ...

The average guinea pig or rat possesses a resistance against typhus infection which llm;ts the distribution of the virus and leads to almost invariable recovery of the animals. Many of our efforts during the last 2 years have focused upon the production of increased susceptibility , in order that we might convert the milder, experimental disease into a fatal one or at least obtain an increased ...

Some chemical and metabolic characteristics of polymorphonuclear leukocytes and monocytes from peritoneal exudates of the guinea pig, and of alveolar macrophages from the same animal, have been compared. Changes in the metabolic patterns of these three types of cell have been followed during the act of phagocytosis. The effect of conventional inhibitors of metabolism, and of anaerobiosis on the...

An in vitro model of cellular immunity in the guinea pig was established. Animals were immunized with tubercle bacilli, bovine gamma globulin, or picrylated human serum albumin in complete Freund's adjuvant. Oil-induced peritoneal exudates from immune and control animals were cultured overnight with and without specific antigen. The cultures were washed and the macrophage monolayers were infect...

A 34-year-old woman with a 3-year history of scleroderma treated with corticosteroids, presented with 2 weeks of abdominal pain and distension and, more recently, nausea. Two months earlier, she had a cesarean section at 29 weeks of gestation for fetal distress. Physical examination showed diffuse abdominal tenderness with signs of peritonitis, and no fever. She had normal white blood cell coun...

Sterile peritoneal exudates produced in rabbits injected with 1% glycogen contain a phospholipase A activity in a cell-free supernatant fraction that hydrolyzed a synthetic phospholipid (1,2-diacyl-sn-glycero-3-phospho-ethanolamine) and phospholipids of autoclaved Escherichia coli. This phospholipase activity (phosphatidylacylhydrolase EC 3.1.1.4) exhibited an apparent bimodal pH optimum (pH 6....

Preparations of pure macrophage suspensions may be required in the investigation of cellmediated immune reactions or for the preparation of antimacrophagic serum. Methods for the preparation of macrophage suspensions of high purity from peritoneal exudates are relatively tedious and result in small yields of cells. Bloom and Bennett (1966) and Turk and Polak (1967) describe a technique inwhich ...

The compositional relationship between the cell surface of rabbit polymorphonuclear leukocytes (PMNs) and the membranes of PMN cytoplasmic granules has been investigated. Heterophilic PMNs obtained from peritoneal exudates contained 13 cell surface polypeptides ranging in molecular weight from 220,000 to 12,000 daltons as determined by lactoperoxidase-catalyzed protein iodination and gel electr...

Mice or rats that have been rendered tolerant of skin homografts from an alien donor strain furnish the basis of a very sensitive and objective test system for investigating the competence of cellular inocula from specifically immunized isologous donors to transfer sensitivity adoptively. By means of this test system it has been shown that immunologically "activated" cells, capable of transferr...