Methylotrophic bacteria comprise a broad range of obligate aerobic microorganisms, which are able to proliferate on (a number of) compounds lacking carbon-carbon bonds. This contribution will essentially be limited to those organisms that are able to utilize methanol and will cover the physiological, biochemical and genetic aspects of this still diverse group of organisms. In recent years much ...

Electroporation conditions were determined for electroporating the broad-host-range plasmid pHX200V-47-ml (22.4 kb) into four pink-pigmented methylotrophic strains (Methylophilus methylotrophus AS1, Methylobacterium extorquens AM1, Methylobacterium organophilum XX, and Pseudomonas sp. M27). For these methylotrophs, a high electric-field strength (15 kV/cm) and high DNA concentration (18.75 ng/~...

s of the XII International Congress of’ Microbiology, Munchen, p. 145. VRDOLJAK, M., MARIC, V. & JOHANIDES, V. (1984). Comparison of the cytochrome content of some methylotrophic bacteria. Periodicum biologorum 86, WIDDOWSON, D. & ANTHONY, C. (1975). The electron transport chain of Pseudomonas AM 1. Biochemical Journal 152, 349-356. WOOD, P. M. (1984). Bacterial proteins with CObinding bor c-ty...

to accomplish the worldwide demand for recombinant human erythropoietin (rhuepo) as a therapeutic, application of cost-efficient expression system of methylotrophic yeast pichia pastoris (p. pastoris) rather than mammalian cells is indispensable. herein, a report on high levels secreted-expression of pichia-derived rhuepo by batch fermentation in a ph stabilized format is presented. the full le...