Oxalate catabolism is conducted by phylogenetically diverse organisms, including Methylobacterium extorquens AM1. Here, we investigate the central metabolism of this alphaproteobacterium during growth on oxalate by using proteomics, mutant characterization, and (13)C-labeling experiments. Our results confirm that energy conservation proceeds as previously described for M. extorquens AM1 and oth...

,YIn a previous study (Sevag and Gots, 1948) on the effects of various drugs on the pneumococcal dehydrogenase systems it was found that, whereas acriflavine, atabrine, propamidine, and optochin could inhibit these systems, high concentrations of sulfonamides had no effect. Other investigators (Mellon aind Bambas, 1937; Brazda and Rice, 1942; Fox, 1942; Clifton and Loewinger, 1943) have reporte...

Objective: To investigate the MTHFD1 G1958A polymorphism involved in the folate metabolism as a risk for head and neck cancer, and to find the association of the polymorphism with the risk factors and clinical and histopathological characteristics. Methods: Retrospective study investigating MTHFD1 G1958A polymorphism in 694 subjects (240 patients in the Case Group and 454 in the Control Group) ...

OBJECTIVE To investigate the MTHFD1 G1958A polymorphism involved in the folate metabolism as a risk for head and neck cancer, and to find the association of the polymorphism with the risk factors and clinical and histopathological characteristics. METHODS Retrospective study investigating MTHFD1 G1958A polymorphism in 694 subjects (240 patients in the Case Group and 454 in the Control Group) ...

The Gly decarboxylase multienzyme complex (Gly cleavage system, EC 2.1.2.10) catalyzes the conversion of Gly into COz, NH3, and 5,10-methylene-tetrahydrofolate, reducing NAD+ to NADH. The enzyme has been isolated from animals, plants, and bacteria-in animals and plants it occurs exclusively in the mitochondrial matrix, and in photosynthetic tissue it can be found in very large amounts (Bourguig...

Most macromolecular X-ray structures are determined from cryocooled crystals, but it is unclear whether cryocooling distorts functionally relevant flexibility. Here we compare independently acquired pairs of high-resolution data sets of a model Michaelis complex of dihydrofolate reductase (DHFR), collected by separate groups at both room and cryogenic temperatures. These data sets allow us to i...

A series of methotrexate (MTX)-resistant human sublines developed by step increases in selected MTX concentrations have been cloned and examined for dihydrofolate reductase (DHFR) content, relative DNA copy number, and sensitivity to MTX. These cloned sublines had increased DHFR levels which were dependent on the presence of MTX in the medium. The increased levels of DHFR in the absence of MTX ...

Abbreviations: ALS: Amyotrophic Lateral Sclerosis; ATP: Adenosine Triphosphate; CBS: Cystathionine β-Synthase; CNS: Central Nervous System; COMT: Catechol-O-Methyl-Transferase; CYP2E1: Cytochrome P450 2E1; eNOS: Endothelial Nitric Oxide Synthase; Hcy: Homocysteine; L-DOPA: Levodopa; mPT: Mitochondrial Permeability Transition; MPTP: 1-Methyl 4-Phenyl 1,2,3,6-Tetrahydropyridine; MTHFR: Methylene ...