alteration in methylation pattern of promoters is associated with tumorigenic events,(1, 2) and differentiation control,(3, 4) in several types of cells in humans.specific hypermethylation in promoter region of some genes exist in different types of neoplastic cells.(5) dna methylation indicates to the addition of a methyl moiety to the cytosine in a cpg dinucleotide. cpg dinucleotides tend to ...

background: the protein of niemann-pick type c1 (npc1) gene promotes the egress of cholesterol from late endosomes and lysosomes to other cellular compartments and contributes to a process known as reverse cholesterol transport. this study aimed to examine whether promoter methylation of npc1 is associated with risk of cardiovascular disease (cvd). methods: fifty cvd patients and 50 healthy sub...

objective: to determine the role of global genome methylation in gastritis lesion and its relation with clinicopathologic finding. materials and methods: the study was conducted on 44 gastritis and normal adjacent specimens using a technique composed of restriction enzyme digestion and pyrosequencing known as luma (luminometric methylation assay). at first, dna extracted from gastritis lesion a...

objective: a recent field of research in epigenetics is dna methylation which involves the cpg island in the genome that subsequently controls transcription and translation of targeted genes. in the hepatitis b virus (hbv) genome, there are three cpg islands which tend to be methylated. the aim of the current study is to determine the methylation pattern of the hbv x gene in chronically infecte...

e-cadherin is among tumor suppressor genes which mostly subjects to the down-regulation in squamous cell carcinoma of esophagus (scce). the gene is tightly associated with the tumor invasion and metastasis in multiple human cancers, especially scce. cpg islands’ methylation in the promoter region of e-cadherin is among the mechanisms that have been suggested for the e-cadherin silencing, howeve...

The p21 belongs to the CIP/KIP family of CDK inhibitors involved in cell cycle arrest at specific stages of the cell cycle progression. DNA methylation is the best studied epigenetic mark that have been evidently associated to chromatin condensation, and repression of gene transcription. The CpG island hypermethylation in promoter region of certain genes occurs in cancer cells and affects tumor...

background: several influential factors such as transcription factors and intracellular signaling components are involved in differentiation of stem cells into a specific lineage. p15 and p16 proteins are among these factors. accumulating evidences has introduced the epigenetic as a master regulator of these factors during lineage specification. the main objective of this study is to determine ...

Background: In somatic cell nuclear transfer (SCNT) method of cloning, transferred nucleus should be dedifferentiated from differentiated state to embryonic state. Molecular analysis showed that the reprogramming in the transferred nucleus was incomplete and cloned embryos have epigenetic abnormalities such as high DNA methylations levels. Since methylation in pre-implantation embryos has a cri...