Methylomonas J is an obligate methylotroph although it is unable to grow on methane. Like Pseudomonas AM1, it produces two blue copper proteins when growing on methylamine, one of which is the recipient of electrons from the methylamine dehydrogenase. When grown on methanol, only the other blue copper protein is produced. We have determined the amino acid sequences of these blue copper proteins...

Previous investigators using the extent of uptake of the weak base methylamine to measure internal pH have shown that the pH in the core region of dormant spores of Bacillus megaterium is 6.3 to 6.5. Elevation of the internal pH of spores by 1.6 U had no significant effect on their degree of dormancy or their heat or ultraviolet light resistance. Surprisingly, the rate of methylamine uptake int...

The Ca(2+)-ATPase in the sarcoplasmic reticulum of skeletal muscle reacts with o-phthalaldehyde (OPA) to form a fluorescent isoindole product. The stoichiometry of labelling of the ATPase is 9 nmol of isoindole/mg of ATPase, corresponding to a 1:1 molar ratio of isoindole: ATPase. There is no evidence for any intermolecular cross-linking. Isoindole formation is faster in the presence of methyla...

In order to improve our understanding of the origin and production processes methylamine in atmosphere its contribution particulate WSON, simultaneous measurements size-segregated aerosols gas phase were conducted at a forested site Yamanashi Prefecture, Japan, over one year. The concentration atmospheric that is sum phase, coarse-mode, fine-mode particles was 0.962 ng-N m−3 on average. About 7...

Computational methods have now become a valuable tool to understand the way in which enzymes catalyse chemical reactions and to aid the interpretation of a diverse set of experimental data. This study focuses on the influence of the condensed-phase environment structure on proton transfer mechanisms, with an aim to understand how C-H bond cleavage is mediated in enzymatic reactions. We shall us...

Semicarbazide-sensitive amine oxidase (SSAO) is an amine oxidase related to the copper-containing amine oxidase family. The tissular form of SSAO is located at the plasma membrane, and is mainly expressed in vascular smooth muscle cells and adipocytes. Recent studies have suggested that SSAO could activate glucose transport in fat cells. In the present work, we investigated the potential role o...

The internal pH of lung lamellar bodies was investigated with membrane permeable basic amines. Isolated granular pneumocytes and isolated lung lamellar bodies exhibited fluorescence when exposed to 8 microM quinacrine, suggesting accumulation of this dye due to an acidic internal pH. Uptake of [14C]methylamine by isolated lung lamellar bodies was measured to quantitate the intralamellar body pH...

1. The following enzymes of the phosphorylated pathway of serine biosynthesis have been found in methanoland succinate-grown Pseudomona8 AM 1: phosphoglycerate dehydrogenase, phosphoserine-a-oxoglutarate aminotransferase and phosphoserine phosphohydrolase. Their specific activities were similar in the organism grown on either substrate. 2. A procedure for preparation of auxotrophic mutants of P...

Archaeal methane formation from methylamines is initiated by distinct methyltransferases with specificity for monomethylamine, dimethylamine, or trimethylamine. Each methylamine methyltransferase methylates a cognate corrinoid protein, which is subsequently demethylated by a second methyltransferase to form methyl-coenzyme M, the direct methane precursor. Methylation of the corrinoid protein re...

Motivated by the recent experiments by Wang et al. (Angew. Chem., Int. Ed. 2012, 51, 6154-6157), in which the alkylamine-capped magic-size (CdSe)13 has been isolated for the first time, we report on the computational modeling of the putative low-lying isomers of (CdSe)13, both bare and ligand-protected. According to Density Functional Theory (DFT) calculations, the core@cage configuration Se@Cd...