Pak choi plants (Brassica rapa ssp. chinensis) were treated with different signaling molecules methyl jasmonate, jasmonic acid, linolenic acid, and methyl salicylate and were analyzed for specific changes in their glucosinolate profile. Glucosinolate levels were quantified using HPLC-DAD-UV, with focus on induction of indole glucosinolates and special emphasis on 1-methoxy-indol-3-ylmethyl gluc...

To create a metabolic sink in the jasmonic acid (JA) pathway, we generated transgenic Nicotiana attenuata lines ectopically expressing Arabidopsis (Arabidopsis thaliana) jasmonic acid O-methyltransferase (35S-jmt) and additionally silenced in other lines the N. attenuata methyl jasmonate esterase (35S-jmt/ir-mje) to reduce the deesterification of methyl jasmonate (MeJA). Basal jasmonate levels ...

To investigate the role of jasmonate in the defense of plants against fungal pathogens, we have studied a mutant of Arabidopsis, fad3–2 fad7–2 fad8, that cannot accumulate jasmonate. Mutant plants were extremely susceptible to root rot caused by the fungal root pathogen Pythium mastophorum (Drechs.), even though neighboring wild-type plants were largely unaffected by this fungus. Application of...

E. Toby Kiers*, Lynn S. Adler, Emily L. Grman and Marcel G. A. van der Heijden Institute of Ecological Science, Vrije Universiteit Amsterdam, De Boelelaan 1085, 1081 HV Amsterdam, The Netherlands; Department of Plant, Soil and Insect Science, University of Massachusetts at Amherst, Massachusetts, USA; Kellogg Biological Station and Plant Biology Department, Michigan State University, Hickory Co...

A blend of ethyl trans-cinnamate, methyl 2-epijasmonate, methyl jasmonate, and (R)-(-)-mellein, identified from the hairpencils of male Oriental fruit moths, attracts sex pheromone-releasing females several centimeters away. The chemicals thereby duplicate the behavioral effect elicited by hairpencil-displaying males during courtship; the chemicals also produce the herbal scent emanating from t...

The purpose of this study was to determine whether methyl jasmonate, a stimulator of Ca -adenosine triphosphatase (ATPase) activity of the purified ATPase from fast-twitch skeletal muscle, could affect contractile responses in small bundles of rat isolated slow-twitch (soleus) fibers. In saponin-skinned fibers, sarcoplasmic reticulum (SR) Ca loading was performed in pCa 7.0 solution. The amount...

Foliar application has been studied to enhance grape composition and, therefore, wine quality. This work examined, for first time, the effects of foliar applications methyl jasmonate (MeJ) and plus urea (MeJ+Ur) Tempranillo vineyard on phenolic, aromatic nitrogen over two vintages (2019 2020). A reduction in alcoholic degree was observed MeJ MeJ+Ur wines. The effect treatments season-dependent....

BACKGROUND Plasma membrane Ca(2+)ATPase is a transport protein in the plasma membrane of cells and helps in removal of calcium (Ca(2+)) from the cell, hence regulating Ca(2+) level within cells. Though plant Ca(2+)ATPases have been shown to be involved in plant stress responses but their promoter regions have not been well studied. RESULTS The 1478 bp promoter sequence of rice plasma membrane...

Shoot–root systemic defense signaling of hybrid poplar (Populus trichocarpa Torr. & A. Gray Populus deltoides Bartr. ex Marsh.) was investigated with molecular techniques to extend existing knowledge of poplar defense. Treatment of roots with methyl jasmonate demonstrated that transcripts of PtdTI3, a poplar trypsin inhibitor and marker of poplar defense responses, can be induced in poplar root...