Methyl green has frequently been used for histochemical and in vitro demonstration of deoxyribonucleic acid (DNA). I t has been reported that this color or staining reaction requires the presence of highly polymeric DNA (1) and that its molecular size influences the extent of the reaction. Thus, as the DNA is broken down by deoxyribonuclease (DNase), there is a corresponding decrease in the bin...

The clinical diagnosis of TRIC-agent infection of the conjunctiva is not difficult for ophthalmologists in endemic areas such as Egypt, but the similarity of the disease to other types of follicular conjunctivitis makes laboratory investigations especially important (Thygeson, I960; Duke-Elder, I965). These include the staining of conjunctival scrapings with Lugol's iodine or Giemsa stain for d...

Of all substances which bulk large in the chemical composition of cellular structures, desoxypentose nucleic acid is perhaps the most clearly demonstrable by application of cytochemical methods to cells. For each one of the three components of nucleotide residues there is a specific method which -enables the cytologist to visualize it on slides: the purines and pyrimidines have natural specific...

Over-reliance on synthetic pesticides in insect pest control has caused widespread public and scientific concerns for human health and the environment, especially since many insect pests have already developed resistances to conventional pesticides and Bt products. For this reason, there is a considerable interest in development of alternative control methods for insect pest management. Based o...

This application note describes a fast analytical method for determination of halosulfuron-methyl using QuEChERS and capillary electrophoresis tandem mass spectrometry (CE/MS/MS). The method was developed and validated for halosulfuron-methyl in green and yellow bell peppers. It showed good linearity (R2 > 0.999) from 5.0 to 500 ppb for the analytical curves prepared in background electrolyte a...

This study reports on the attractiveness of volatile chemicals to green lacewings (Neuroptera: Chrysopidae) and flower flies (Diptera: Syrphidae) as measured by catch on yellow sticky traps within corn [Zea mays L. (Cyperales: Poaceae)] and soybean [Glycine max (L.) Merr. (Fabales: Fabaceae)] plots. Green lacewings were attracted to eugenol-baited traps in two tests in soybean plots. Follow-up ...

Pectin methyl esterases (PMEs) and their endogenous inhibitors are involved in the regulation of many processes in plant physiology, ranging from tissue growth and fruit ripening to parasitic plant haustorial formation and host invasion. Thus, control of PME activity is critical for enhancing our understanding of plant physiological processes and regulation. Here, we report on the identificatio...

The increasing interest in the development of novel green solvents has led to the synthesis of benign alternative products with minimized environmental impacts. However, most of published studies on green solvents focus primarily on their physicochemical properties, with limited emphasis on absence of ecotoxicological assessment. In this study, we evaluated the acute ecotoxicity of four levulin...

Antimutagenic activity of green tea (Camellia sinensis) was studied using Salmonella typhimurium strains (TA 102) (Ames test). Aqueous tobacco extract was found to be mutagenic to S. typhimurium TA 102 at concentration of 50 mg/plate. Green tea polyphenols was found to inhibit the mutagenicity of tobacco in a concentration-dependent manner. Concentrations needed for 50% inhibition of mutagen-in...

Walnut, Juglans regia L., is known for its insecticidal activities to a range of herbivores and microbes. Isolation and identification of bioactive compounds from walnut is a potential approach for the development of new pesticides. Laboratory experiments were carried out to investigate the acaricidal activities of green husk extracts of walnut. Bioassay-guided fractionation of petroleum-ether ...