Increased production of penicillin-binding protein PBP 4 is known to increase peptidoglycan cross-linking and contributes to methicillin resistance in Staphylococcus aureus. The pbp4 gene shares a 400-nucleotide intercistronic region with the divergently transcribed abcA gene, encoding an ATP-binding cassette transporter of unknown function. Our study revealed that methicillin stimulated abcA t...

The use of antibiotics in farm management (growing crops and raising animals) has become a major area of concern. Its implications is the consequent emergence of antibiotic resistant bacteria (ARB) and accordingly their access into the human food chain with passage of antibiotic resistance genes (ARG) to the normal human intestinal microbiota and hence to other pathogenic bacteria causative hum...

The MRSA screen test (Denka Seiken Co., Ltd.), a commercially available, rapid (20-min) slide latex agglutination test for the determination of methicillin resistance by detection of PBP 2a in Staphylococcus aureus, was compared with the oxacillin agar screen test and PCR detection of the mecA gene. A total of 563 S. aureus isolates were tested. Two hundred ninety-six of the isolates were methi...

BACKGROUND The treatment of methicillin-resistant staphylococcal infections has been a growing problem both in and out of hospitals for the past 30 years. Therefore, there is a need for other antibiotics as an alternative to glycopeptides in the treatment of methicillin-resistant staphylococcal infections. This study investigated the in vitro susceptibility of 49 methicillin-resistant Staphyloc...

background pyogenic bacteria and especially staphylococcus aurous (s. aurous) are the most common cause of chronic osteomyelitis. not only treatment protocol of chronic osteomyelitis occasionally is amiss but also this malady responds to treatment difficultly. objectives this study investigates antibiotic resistance pattern of s. aurous isolated from iranian patients who suffer from chronic ost...

background methicillin-resistant staphylococcus aureus (mrsa) remains a significant public health problem and treatment challenge. objectives this study was conducted to determine the frequency, molecular types, and drug resistance of s. aureus isolated from nasal carriers in two teaching hospitals (hajar and kashani) in shahrekord, southwestern iran. methods in this cross-sectional study, 262 ...

Characteristic for methicillin-resistant (Mcr) staphylococci is the heterogeneous expression of the intrinsic methicillin resistance. The majority of the cells express resistance to low concentrations of methicillin, and a minority of the cells express resistance to much higher concentrations. We show here (i) that the presence of the mecA encoding region on plasmid pBBB79 was sufficient to ren...

Mupirocin is a topical antimicrobial agent utilized in treatment of methicillin resistant Staphylococcus aureus. Mupirocin resistance in Staphylococcus aureus is increasingly being reported in many parts of the world in the context of its widespread use. The objectives of this study were to determine the susceptibility of methicillin resistant Staphylococcus aureus clinical isolates to mupiroci...

BACKGROUND & OBJECTIVES Methicillin resistant Staphylococcus aureus (MRSA) isolates with inducible clindamycin resistance (iCR) are resistant to erythromycin and sensitive to clindamycin on routine testing and inducible clindamycin resistance can only be identified by D-test. This study was aimed to detect methicillin resistance and iCR among S. aureus isolates, effectiveness of some commonly u...

It was shown earlier that Tn551 inserted into the C-terminal region of murE of parental methicillin-resistant Staphylococcus aureus strain COL causes a drastic reduction in methicillin resistance, accompanied by accumulation of UDP-MurNAc dipeptide in the cell wall precursor pool and incorporation of these abnormal muropeptides into the peptidoglycan of the mutant. Methicillin resistance was re...