Cyclosporine A-treated transplant recipients develop pronounced cardiovascular disease and have increased oxidative stress and altered antioxidant capacity in erythrocytes and plasma. These experiments investigated the time-course of cyclosporine A-induced changes to redox balance in plasma and erythrocytes. Rats were randomly assigned to either a control or cyclosporine A-treated group. Treatm...

aldose reductase is a critical enzyme in the polyol pathway that plays an important role in diabetes mellitus. inhibition of the activity of this enzyme can prevent cataract in diabetic patients’lenses. in this study the inhibitory effect of two flavonoids, quercetin and naringin, in the activity of aldose reductase in streptozotocin-induced diabetic and healthy rats were investigated. thirty m...

background: the association between methylene tetrahydrofolate reductase polymorphism and coronary artery diseases risk has been both confirmed and refuted in a number of published studies. the aim of this study was to investigate whether genetic polymorphisms of mthfr (c677t, a1298c) contributed to the development of myocardial infarction (mi). materials and methods: the present case-control s...

Objective(s): Kisspeptin and opioids are important factors for controlling GnRH/LH secretion. In present study, influences of kisspeptin or morphine were investigated on 5α- reductase or aromatase (CYP19) genes expression in the hypothalamus, testis and liver. It aimed to investigate whether kisspeptin pathway may control morphine effects on plasma concentration of tes...

In the preceding paper, Keilin & Wang (1946) have described the preparation and properties of the haemoglobin obtained from the tracheal cells of the larva of Gastrophilus (the bot fly). Samples from three of their preparations in the form ofmethaemoglobin were used for measurements of osmotic pressure. The amount of material available was small and the results recorded must be regarded as prov...

Haemoglobin (Hb)-M Hyde Park, also known as Hb-M Akita is a rare type of hereditary Hb M due to autosomal dominant mutation of CAC>TAC on codon 92 of β globin gene resulting in the replacement of histidine by tyrosine on β globin chain. This variant Hb has a tendency to form methaemoglobin (metHb). The iron ion in metHb is oxidized to ferric (Fe3+) which is unable to carry oxygen and the patien...

AIM To assess the safety and efficacy of EMLA cream (eutectic mixture of local anaesthetics) used to induce surface anaesthesia for venepuncture in healthy preterm infants. METHODS Nineteen infants, median gestational age 31 weeks (range 26-33 weeks) were assessed in a randomised, double blind, placebo controlled, cross-over trial. Changes in physiological variables (heart rate, blood pressur...

background anaerobic-inducible promoters are alternatives of chemical-inducible promoters for expression of recombinant proteins especially in conditions where chemical induction is not possible or anaerobic conditions are preferable. the nirb promoter is the promoter of the first gene of nir operon in escherichia coli, which encodes nadh-dependent nitrite reductase. this promoter is naturally ...

Cyanosis is a physical finding that can occur at any age but presents a great challenge as the causes are multiple and varied. When patients present with cyanosis and dyspnoea that are unrelated to cardiopulmonary causes, methaemoglobinaemia should be considered as a possible diagnosis although rare. Methaemoglobinaemia can be asymptomatic even when methaemoglobin (metHb) levels are as high as ...

THE oxyhaemoglobin content of blood can be rapidly and accurately estimated with the help of a photoelectric colorimeter. In the absence of oxyhaemoglobin or of other pigments, methaemoglobin can be determined in the same way. In whole blood the presence of metor of sulph-haemoglobin or of other pigments can be detected. The experiments also indicate that the use of 084% ammonia as a diluent fo...