BACKGROUND Oocyte cryopreservation is an important part of modern fertility treatment. The effect of vitrification on the fertilization and developmental rates of embryo is still a matter of debate. OBJECTIVES This study aimed to investigate the effect of vitrification on the success of mouse oocyte maturation, fertilization, and preimplantation development in vitro. MATERIALS AND METHODS I...

BACKGROUND Imprinted genes, many of which are involved in development, are marked during gametogenesis to allow their parent-of-origin specific expression, and DNA methylation at CpG islands is part of this epigenetic mark. Maternal imprint is apposed on oocyte during growth and maturation. Factors interfering with normal oocyte differentiation such as gonadotrophin stimulation and in vitro mat...

The recovery of immature oocytes followed by in vitro maturation, fertilization and culture (IVMFC) allows the rescue of biological material of great genetic value for the establishment of genetic resource banks of endangered species. Studies exist on sperm cryopreservation of endangered Mohor gazelle (Gazella dama mhorr), but no work has been carried out yet on oocyte collection, fertilization...

In mammals, female meiosis consists of two asymmetric cell divisions, which generate a large haploid oocyte and two small polar bodies. Asymmetric partitioning of the cytoplasm results from migration of the meiotic spindle toward the cortex and requires actin filaments. However, the subcellular localization and the role of the existing two cytoplasmic actin (CYA) isoforms, beta and gamma, have ...

Background: Oocyte cryopreservation is one of the most important topics in the field of assisted reproductive technology to preserve women fertility, but relationship between cryopreservation and apoptosis is still a matter of debate. The present study was aimed to investigate the effects of vitrification on apoptosis in mouse oocytes by Cryotop method. Method: A total of 200 germinal vesicle (...

Background: Polycystic ovary syndrome (PCOS) is characterized by chronic anovulation and hyperandro genism and affects approximately 5-10% of women of reproductive age. In vitro maturation (IVM) of mammalian oocytes has become an efficient method to produce mature oocytes in order to use in assisted reproductive techniques such as in vitro fertilization (IVF), intra cytoplasmic sperm injection ...

Oocyte-specific genes play important roles in regulating ovarian development, principally through the proper and timely progression of oogenesis and folliculogenesis. Development of transgenic mouse models has been critical in revealing how oocyte-specific transcripts influence oocyte development and growth, integrity of the oocyte-granulosa cell complex, oocyte maturation, fertilization, and e...

OBJECTIVE To better characterize cumulus-oocyte interactions during oocyte maturation and fertilization in the cat model. DESIGN Experimental in vitro study. SETTING Smithsonian Institution. ANIMAL(S) Domestic shorthair cats. INTERVENTION(S) Groups of denuded oocytes (DOs) and cumulus-oocyte complexes (COCs) were subjected to in vitro maturation (with or without FSH and LH, with or with...

BACKGROUND During oocyte maturation in Xenopus, progesterone induces entry into meiosis I, and the M phases of meiosis I and II occur consecutively without an intervening S phase. The mitogen-activated protein (MAP) kinase is activated during meiotic entry, and it has been suggested that the linkage of M phases reflects activation of the MAP kinase pathway and the failure to fully degrade cycli...

objective: cryopreservation of ovarian tissue or follicles has been proposed as an alternative method for fertility preservation. although successful vitrification of follicles has been reported in several mammalian species, the survival rate is generally low. the aim of this study was to investigate the effects of fibroblast growth factor (fgf) and epidermal growth factor (egf) on in vitro pre...