In this work, we evaluated antioxidant and metal chelating activities of common bean proteins. Protein fractions were produced by using extraction with organic (method 1) or aqueous (method 2) solvents. Results evidenced that the amount of proteins extracted through method 2 was higher than method 1, probably due the chemical difference between solvents. The antioxidant activity of hard-to-cook...

Wilson disease ATPase (ATP7B) has been implicated in the resistance of cancer cells to cisplatin. Using a simple in vivo assay in bacterial culture, in the present study we demonstrate that ATP7B can confer resistance to cisplatin by sequestering the drug in its N-terminal metal-binding domain without active drug extrusion from the cell. Expression of a protein fragment containing four N-termin...

The human MDR1 gene is induced by cadmium exposure although no resistance to this metal is observed in human cells overexpressing hMDR1. To access the role of MDR proteins in cadmium resistance, human MDR1, Lactococcus lactis lmrA, and Oenococcus oeni omrA were expressed in an Escherichia coli tolC mutant strain which proved to be hypersensitive to cadmium. Both the human and bacterial MDR gene...

BACKGROUND While copper has essential functions as an enzymatic co-factor, excess copper ions are toxic for cells, necessitating mechanisms for regulating its levels. The cusCBFA operon of E. coli encodes a four-component efflux pump dedicated to the extrusion of Cu(I) and Ag(I) ions. METHODOLOGY/PRINCIPAL FINDINGS We have solved the X-ray crystal structure of CusC, the outer membrane compone...

This article reviews the infrequently utilized treatment method of forced eruption and how it can serve as an alternative to the sacrifice of the natural root system. Forced eruption can preserve the natural root system and related periodontal architecture, resulting in years of additional service for the patient. It also can maintain adjacent tooth structure while retaining the option for futu...

Sheet and film processes include polymer film extrusion, coating processes of many types, paper manufacturing, sheet metal rolling, and plate glass manufacture. Identification, estimation, monitoring, and control of sheet and film processes are of substantial industrial interest since effective control means reduced usage of raw materials, increased production rates, improved product quality, e...

The article presents the results of development and research various technological schemes process stamping by rolling cylindrical conical rolls to obtain complex profile workpieces. It is shown that achievement significant dimensions elements workpiece possible providing a directed flow metal changing relative position roll workpiece. most effective operations are landing, settling, unloading,...

A new type of material, a "nanobursa" mesh (from "bursa" meaning "sac or pouch"), is introduced. This material consists of sequential layers of porous polymeric nanofibers encapsulating carbon nanotubes, which are functionalized with different metal nanoparticles in each layer. The nanobursa mesh is fabricated via a novel combination of twin-screw extrusion and electrospinning. Use of this hybr...

The usage of the finite volume method (FVM) for calculation of bulk metal forming working pressure is described in this paper. FVM is applied on two-dimensional orthogonal grids and colocated variable arrangement. The application of the FVM is done in case of solving the plastic flow during extrusion processes with different boundary conditions. The method of involving of the most frequently us...