Micro-vascular complications such as diabetic nephropathy (DN) develop in patients with uncontrolled T1DM. Low density lipoprotein receptor-related protein 5 (LRP5) is the co-receptor ligand of Wnt signaling pathway. Many single nucleotide variants have been identified human LRP5 gene and were reported to be associated metabolic disorders. The Wnt/β-catenin pathway has proposed play an importan...

This paper presents an in silico optimization method of metabolic pathway production. The metabolic pathway can be represented by a mathematical model known as the generalized mass action model, which leads to a complex nonlinear equations system. The optimization process becomes difficult when steady state and the constraints of the components in the metabolic pathway are involved. To deal wit...

MOTIVATION Pathway Hunter Tool (PHT), is a fast, robust and user-friendly tool to analyse the shortest paths in metabolic pathways. The user can perform shortest path analysis for one or more organisms or can build virtual organisms (networks) using enzymes. Using PHT, the user can also calculate the average shortest path (Jungnickel, 2002 Graphs, Network and Algorithm. Springer-Verlag, Berlin)...

Iguratimod is a new synthetic disease-modifying antirheumatic drug intended to treat patients with rheumatoid arthritis. A new method using recombinant human CYP450s yeast cells containing c-DNA expressed P450s was applied to identify the metabolic pathways of iguratimod and to prepare its metabolite. The metabolite was isolated, and its structure was identified by quadrupole time-of-flight-mas...

Mycobacterium tuberculosis, the main cause of tuberculosis (TB), has still remained a global health crisis especially in developing countries. Tuberculosis treatment is a laborious and lengthy process with high risk of non compliance, cytotoxicity adverse events and drug resistance in patient. Recently, there has been an alarming rise of drug resistant in TB. In this regard, it is an unmet need...

This article is aim to investigate the functional role and potential regulatory mechanisms of solute carrier family 35 member D3 (SLC35D3) in colorectal cancer. Beyond analyzing databases, western blot was used detect verify expression SLC35D3. Cell proliferation invasion or migration ability were detected by CCK-8, EdU, Transwell assays. Animal experiments conducted if biological function SLC3...

MOTIVATION There is an imperative need to integrate functional genomics data to obtain a more comprehensive systems-biology view of the results. We believe that this is best achieved through the visualization of data within the biological context of metabolic pathways. Accordingly, metabolic pathway reconstruction was used to predict the metabolic composition for Medicago truncatula and these p...