UNLABELLED The integrity of onion cells and its impact on tissue texture after high pressure and thermal processing was studied. The contribution of cell membranes and the pectic component of cell walls on the texture properties of onion tissue were analyzed. Neutral red (NR) staining of onion parenchyma cell vacuoles was used for the evaluation of cell membrane integrity and microscopic image ...

Background: One of the factors limiting successful processing of alpaca (Vicugna pacos) semen is the viscosity of seminal plasma. The viscous nature of the collected ejaculate has hindered sperm cryopreservation as well as artificial insemination (AI) under field conditions. Aims: The objective of this investigation was to evaluate recovery, motility, ...

Semen samples from 9 sexually mature dogs of different breeds, aged from 1 to 5 years were used. Quality of sperm was evaluated using the following parameters: progressive motility (subjective), then structural and functional integrity of spermal membrane (supravital colouring (eosine/nigrosine) and HOS test). The majority of HOS reactive or swollen spermatozoa was placed in a hypoosmolar solut...

this experiment was conducted to evaluate the addition of seminal plasma to coated spermatozoa after 72 h. semen was collected from four taleshi rams by artificial vagina. to prepare coated spermatozoa, semen was collected in tube containing tris-fructose-15% egg yolk. after initial evaluation, the samples were mixed and centrifuged. after removal of supernatant and dilution, samples were divid...

this study was conducted to evaluate the effect of egg yolk and cooling on ram coated spermatozoa. semen was collected from three ram by artificial vagina contacted with a tube containing tris- fructose-egg yolk 15%. samples were pooled, centrifuged by 700 g for 10 min and removed supernatant. then, samples were diluted by tris-glucose and centrifuged again to remove seminal plasma and egg yolk...

the effect of osmolarity and glycerol different levels in soybean lecithin-based extender on the bull sperm quality after cryopreservation was examined using six holstein bulls in a 2 × 3 factorial trial based on completely randomized design, with three levels of osmolarity (250, 300 and 350 mosml) and two levels of glycerol (5 and 7 %) .in general, semen samples were collected 36 times (from e...

the anti-senescence biogenic polyamines (pas) putrescine (put), spermidine (spd) and spermine (spm) are involved in plant growth processes and responses to stress. the physiological mechanism of exogenous put to affect the senescence of cut lisianthus flowers during vase life was investigated. fresh cut lisianthus flowers were treated with distilled water (control), 0.5, 1 and 2 mm put and then...

Background: Sperm cryopreservation-thawing process has damaging effects on the structure and function of sperm, namely cryoinjury. Calcium overload has been reported as a postulated mechanism for sperm damage during the first steps after thawing. This study was designed to assess the intracellular calcium (Ca2+i) after cryopreservation and to clarify the role of a calcium chelator ethylene glyc...

Antioxidants partially ameliorated the negative effects of reactive oxygen species (ROS) produced during cryopreservation. The objective of the present study was to investigate the effect of reduced glutathione, ascorbic acid and α-tocopherol on the quality of frozen-thawed buck spermatozoa. Ejaculates were collected by artificial vagina from 8 bucks and microscopically evaluated at 37 0 C. The...