purpose : to investigate the effects of mtp-131 on trabeculum cells cultured under hydrostatic pressure methods : glaucomatous human trabecular meshwork (gtm3) cells were divided into three groups: the mtp-131 group, the hydrostatic group, and the normal group. the mtp-131 group was pretreated with 1 µm mtp-131, and the cells were then cultured under 80 mmhg pressure for 24 hours. the normal gr...

The molecular mechanisms and forces involved in the translocation of bacterial toxins into host cells are still a matter intense research. adenylate cyclase (CyaA) toxin from Bordetella pertussis displays unique intoxication pathway which its catalytic domain is directly translocated across target cell membranes. CyaA region contains segment, P454 (residues 454–484), exhibits membrane-active pr...

Background: Heart failure is one of the most common cardiovascular disorders and is considered a chronic, progressive and debilitating disorder. The medical treatment of this disease is accompanied by many problems. Today, stem cells are being used increasingly to reduce the problems of heart failure treatments. Since pro-inflammatory cytokines play an important role in the prognosis and progre...

introduction perforation of maxillary sinus mucous membrane is of the most prevalent complication during open sinus lift surgery. moreover, such complication can usually be managed by an absorbable membrane. as far as absorbable membranes are concerned, decellularized maxillary sinus mucous membranes, which is an extracellular matrix, can be used as a biologic scaffold and insulating membrane i...

objective(s): this study was designed to indicate whether different fractions from artemisia biennis hydroethanolic extract could provide cytoprotection against oxidative stress and apoptosis induced by doxorubicin (dox) in rat pheochromocytoma cell line (pc12). material and methods:cell viability was determined by mtt assay. also, activation of caspase-3 and superoxide dismutase were evaluated...

purpose: to develop a nanofibrous polycaprolacton (pcl) substrate for limbal stem cell (lsc) expansion that can serve as a potential alternative substrate to replace human amniotic membrane. methods: the human limbal stem cells (lscs) were used to evaluate the biocompatibility of substrates (nanofibrous scaffold, and human amniotic membrane) regarding cellular phenotypic profile, viability, pro...

Proton exchange membrane fuel cells (PEMFCs) are electrochemical devices that show the highest power densities compared to the other type of fuel cell. In this work, nanocomposite membranes used for proton exchange membrane fuel cells as poly(vinyl alcohol)/La2Ce2O7 (PVA-LC) with the aim of increasing the water uptake and proton conductivity. Glutaraldehyde (GA) was used as cross linking agent ...

Introduction: Electroporation is a practical technique used to transport the molecules across the cell membrane. The utilization of fluorescent molecules is the method widely used to evaluate the electropermeability of cell membrane as a result of pulse application. It is also possible to use mathematical methods to predict the changes caused in cell electropermeability as a result of the chang...

Vapor nanobubble-mediated photoporation has evolved into a promising physical intracellular delivery technology. When irradiated with short but intense laser pulses, photothermal nanomaterials can generate vapor nanobubbles that, when they collapse, induce transient membrane pores through which exogenous effector molecules be delivered the cells. Interestingly, this technique offers high-throug...