The effect of activation and overexpression of the nuclear receptor PPAR-β/δ in human MDA-MB-231 (estrogen receptor-negative; ER(-)) and MCF7 (estrogen-receptor-positive; ER(+)) breast cancer cell lines was examined. Target gene induction by ligand activation of PPAR-β/δ was increased by overexpression of PPAR-β/δ compared with controls. Overexpression of PPAR-β/δ caused a decrease in cell prol...

To investigate the role of AP-1 transcription factors in mediating retinoid-induced growth suppression of breast cells, we studied the sensi tivity of MCF7 breast cancer cells with different levels of AP-1 activity to all-frans retinole acid (atRA). AP-1 activity was increased in MCF7 cells by stably transfecting c-jun cDNA into these cells. Parental and vectortransfected MCF7 cells, which were...

INTRODUCTION Phoenix dactylifera L (Date palm) is a native plant of the Kingdom of Saudi Arabia (KSA) and other Middle Eastern countries. Ajwa date has been described in the traditional and alternative medicine to provide several health benefits including anticholesteremic, antioxidant, hepatoprotective and anticancer effects, but most remains to be scientifically validated. Herein, we evaluate...

Ubiquitin carboxy terminal hydrolase-L1 (UCH-L1) belongs to the UCH proteases family that deubiquitinates ubiquitin-protein conjugates in the ubiquitin-proteasome system. Previous research showed that UCH-L1 was expressed in mouse retinal cells and testicular germ cells, and its function was associated with apoptosis. But it is still unclear whether UCH-L1 is concerned with apoptosis in tumor c...

Our previous studies have shown that bee venom (BV) can induce apoptosis in human cervical cancer Ca Ski cells, but it can also affect human breast cancer cells, though its molecular mechanisms are not precisely known. In this study, the molecular mechanisms of apoptosis induced by BV in human breast cancer MCF7 cells were investigated. BV induced morphological changes (examined by phase-contra...

Cancer-associated fibroblasts (CAFs) play a role in tumour initiation and progression, possibly by inducing epithelial-to-mesenchymal transition (EMT), a series of cellular changes that is known to underlie the process of metastasis. The aim of this study was to determine whether CAFs and surrounding normal breast fibroblasts (NBFs) are able to induce EMT markers and functional changes in breas...

Abstract Estrogen receptor positive (ER+) breast cancer is the most common subtype among all cancers and responsible for related deaths. Endocrine therapies, such as selective estrogen modulators (SERM) aromatase inhibitors (AI), often given in combination with cyclin-dependent kinase 4/6 (CDK4/6) inhibitors, are initially effective but majority of advanced ER+ eventually become refractory to e...

Chemical investigation of Garcinia eugenifolia and Calophyllum enervosum yielded six compounds. One of these was found to be a novel compound identified as enervosanone. Five known compounds cambogin, epicatechin, osajaxanthone, rubraxanthone and isocowanol, were also isolated. These compounds were tested for their bioactivity as antimicrobial, antioxidant and cytotoxicagents. Antimicrobial...

s and Poster Presentations Sankala H, Hait NC, Paugh SW, Milstien S, Spiegel S. Sphingosine kinase 2 regulates doxorubicininduced cell cycle arrest and p21 independently of p53 in MCF7 cells. 22nd Annual Daniel T. Watts Research Poster Symposium, Virginia Commonwealth University, Richmond, VA. October 25-26, 2005. Sankala H, Hait NC, Paugh S, Milstien S, Spiegel S. The role of sphingosine kinas...