The aim of the present study was to establish a tamoxifen-resistant cell line (MCF-7/TAM-R) and to investigate the therapeutic effect of G47Δ on this cell line both in vitro and in vivo. In the present study, the MCF-7/TAM-R monoclonal subline was established after exposing MCF-7 cells to tamoxifen for 21 days. Then, it was compared with a wild-type MCF-7 subline (MCF-7W), which was not treated...

Multidrug-resistant cancer cells display elevated levels of glucosylceramide (Lavie, Y., Cao, H. T., Volner, A., Lucci, A., Han, T. Y., Geffen, V., Giuliano, A. E., and Cabot, M. C. (1997) J. Biol. Chem. 272, 1682-1687). In this study, we have introduced glucosylceramide synthase (GCS) into wild type MCF-7 breast cancer cells using a retroviral tetracycline-on expression system, and we develope...

A variant of the breast cancer MCF-7 cells, termed MCF-7/SNR, was established by chronic and step-wise exposure to SN38, an active metabolite of irinotecan. MCF7/SNR cells were >100-fold more resistant to SN38 than parental MCF-7 cells, while resistances to vincrisitine and cisplatin were similar between those cells. MCF-7/SNR cells overexpressed endogenous breast cancer resistance protein (BCR...

Background: Traditional medicine is inspiring in drug development research. Epilobium parviflorum extracts have shown promising therapeutic effects on prostate cancer cells. The similarities between breast and prostate cancers at molecular and metabolic levels prompted us to explore its effects on human breast cancer. Methods: The root, aerial parts and flowers of the plant were, collected and...

Even though a tremendous number of multifunctional nanocarriers have been developed to tackle heterogeneous cancer cells, little attention has been paid to elucidate how to rationally design a multifunctional nanocarrier. In this study, three individual functions (active targeting, stimuli-triggered release and endo-lysosomal escape) were evaluated in doxorubicin (DOX)-sensitive MCF-7 cells and...

High mobility group-1 (HMGB-1) enhances the DNA interactions and possesses a transcriptional activation potential for several families of sequence-specific transcriptional activators. In order to examine the effect of HMGB-1 on the cell cycle progression in MCF-7 cells, the HMGB-1 expression vector was transfected into synchronized MCF-7 cells, and the effect of HMGB-1 overexpression on the cel...

It is known that the ovarian extract of Singkarak Lake Pufferfish (Tetraodon leiurus) has potential for chemoprevention in MCF-7 cells. However, expression Bcl-2 and Bax genes was associated with cancer. Therefore, this study aimed to analyze gene induced by MCF-7. The method an vitro experiment using control cells leiurus), Real-time PCR used examine genes. result showed had insignificant decr...

Background: Molecular targeted therapy by different cell death inducers are recently considered in cancer therapy. The aim of this study was to compare the effect of cisplatin and inositol trisphosphate kinase inhibitor (caffeine) on human breast cancer cell line (MCF-7). The pattern of cell death in MCF-7 cells following the exposure to cisplatin and caffeine in individual and combination form...