A high-resolution fluorescence microscopy technique has been developed that achieves a lateral resolution of better than one sixth of the emission wavelength (FWHM). By use of a total-internal-reflection geometry, standing evanescent waves are generated that spatially modulate the excitation of the sample. An enhanced two-dimensional image is formed from a weighted sum of images taken at differ...

We report on the experimental demonstration of detuned-resonator induced transparency in the near-infrared (~800 nm) using two detuned racetrack resonators side-coupled to a bus waveguide. Both resonators and the bus waveguide are in the form of dielectric-loaded surface plasmon polariton waveguides. Leakage radiation microscopy imaging is employed to measure transmission spectra, featuring loc...

Simplicity and low cost has positioned inkjet paper- and fabric-based 3D substrates as two of the most commonly used surface-enhanced Raman spectroscopy (SERS) platforms for the detection and the identification of chemical and biological analytes down to the nanogram and femtogram levels. The relationship between far-field and near-field properties of these 3D SERS platforms remains poorly unde...

We describe a new approach to making luminophores that display long emission wavelengths, long decay times, and high quantum yields. These luminophores are covalently linked pairs with a long-lifetime resonance-energy-transfer donor and a long-wavelength acceptor. The donor was a ruthenium (Ru) metal-ligand complex. The acceptor was the Texas Red. The donor and acceptor were covalently linked b...

Two kinds of aequorin-type photoproteins, i.e., halistaurin and phialidin, and four kinds of modified forms of aequorin, i.e., products of acetylation, ethoxycarbonylation, fluorescamine-modification and fluorescein labelling, were prepared. The modified forms of aequorin were more sensitive to Ca2+ than was aequorin in their Ca2+-triggered luminescence reactions, whereas halistaurin and phiali...

We have detected sub-wavelength spot sizes in the near-field of a metal tip, which is illuminated with terahertz (THz) pulses. The THz near-field is detected by using electro-optic detection in a (100) oriented GaP crystal. Contrary to conventional electro-optic detection, which uses (110) oriented detection crystals, (100) crystals only allow the detection of THz light, polarized perpendicular...

The DNA mediated assembly of complementary DNA-functionalized gold nanoparticles (DNA-AuNP) was investigated by means of UV/Vis-spectroscopy and Dynamic Light Scattering (DLS). The melting temperature of the aggregates was determined to be T(m) = 31 degrees C. Characterization of the assembly at 20 degrees C, 25 degrees C and 30 degrees C showed a decrease of the initial assembly growth rate wi...

A quantitative interrelation between the physical parameters of electromagnetic and acoustic waves perceived by the sight and hearing organs of a human has been discovered. It is shown that visible light wavelengths can be correlated with the range most used by humans for acoustic communication, including, in particular, verbal dialogue. It is shown, that maxima of spectroscopic sensitivity of ...

Using scanning tunneling microscopy and spectroscopy, we have studied the local density of states (LDOS) of graphene over step edges in boron nitride. Long-wavelength oscillations in the LDOS are observed with maxima parallel to the step edge. Their wavelength and amplitude are controlled by the energy of the quasiparticles allowing a direct probe of the graphene dispersion relation. We also ob...

Errors in the double variation teclinique of refractive index measurement are analyzed using a new approach. The ability to measure matching wavelength is characterized, along with the effect on the calculated refractive index. Refractive index accuracy and precision are very dependent on the specifics of each calibration set, particularly the difference in dispersion between the liquid and sol...