The random amplified polymorphic DNA (RAPD) technique has been used widely for purposes such as the construction of linkage maps, QTL analysis, evaluation of genetic diversity, and parentage tests. However, some minor bands of RAPD display a low reproducibility and low reliability. We describe a simple and efficient method for making minor RAPD bands clearer. The first step of this method is to...

MAS (marker assisted selection) is useful for cultivar development in the lower plant Saccharina japonica . In this study, 167 screened SSR markers and 125 individuals were used to construct a S.japonica genetic linkage map with 27 groups total Haldane length of 2069 cM. Constructed aligned assembled pseudo-chromosomes S. genome sequences (NCBI: MEHQ00000000) anchored 23 31 pseudo-chromosomes. ...

Many agriculturally important traits are complex, affected by many genes and the environment. Quantitative trait loci (QTL) mapping is a key tool for studying the genetic structure of complex traits in plants. In the present study QTLs associated with yield and agronomical traits such as leaf number, leaf length, leaf width, plant height, stem and head diameter were identified by using 70 recom...

the growth hormone receptor (ghr) is a member of cytokine/hematopoietin family that mediates the biological actions of growth hormone (gh) on target tissues. therefore, the purpose of this study was to examine the association of tg-repeat polymorphisms in the 5’-flanking region of bovine ghr gene with milk production traits and somatic cell score (scs) in holstein cattle of iran. the part of 5’...

in this study, 100 moghani sheep from breeding station located in jaafarabad, moghan were genotyped for exon 1 of igf-i gene, using pcr-sscp. different band patterns were obtained in this site gene that was studied. based on sscp patterns, three genotypes bc, bd and bb obtained with frequencies of 65%, 26% and 9%, respectively. the genotype frequencies had a significant deviation from hwe. in a...

body weight (bw) is a complex and important economic trait that may benefit from the implementation of marker assisted selection (mas). the objective followed in this study was to identify qtls associated with bw at different ages as distinct traits based on an experimental half-sib cross of japanese quails divergently selected for bw at 4 weeks of age. the body weights at hatch, and as well at...

Many commercial inbred lines are available in crops. A large amount of genetic variation is preserved among these lines. The genealogical history of the inbred lines is usually well documented. However, quantitative trait loci (QTL) responsible for the genetic variances among the lines are largely unexplored due to lack of statistical methods. In this study, we show that the pedigree informatio...

Seed calcium content is an important quality attribute of specialty soybean [Glycine max (L.) Merr.] for soyfoods. However, analyzing seed for calcium content is time consuming and labor intensive. Knowing quantitative trait loci (QTL) for seed calcium will facilitate the development of elite cultivars with proper calcium content through marker-assisted selection (MAS). The objective of this st...

Eucommia ulmoides is cultivated for the production of eucommia rubber and Chinese herbal drugs. Molecular breeding methods, such as marker-assisted selection (MAS), have the potential to improve the efficiency of E. ulmoides breeding. Quantitative trait locus (QTL) analysis was applied to identify marker-trait associations for E. ulmoides using an F1 mapping population of 152 individuals derive...

It is of significance to discover genes related to fiber quality and yield traits and tightly linked markers for marker-assisted selection (MAS) in cotton breeding. In this study, 188 F8 recombinant inbred lines (RILs), derived from a intraspecific cross between HS46 and MARCABUCAG8US-1-88 were genotyped by the cotton 63K single nucleotide polymorphism (SNP) assay. Field trials were conducted i...