objective: to study the mechanisms involved in amyloid formation processes, we made a mammalian cell culture model of amylin aggregation and characterized its properties. materials and methods: amyloid fibrils were extracted from cho cells and binding affinity to thioflavin t and congo red was investigated. then the apple-green birefringence of extracted fibrils was detected under polarized li...

Neural stem cells (NSCs) as a heterogeneous multipotent and self- renewal population are found in different areas in the developing mammalian nervous system, as well as the sub-ventricular zone (SVZ) and the hippocampus of the adult brain. NSCs can give rise to neurons, astrocytes and oligodendrocytes. The aim of this study was to differentiate neural stem cells into noradrenergic–like cells in...

Understanding cell fate patterning and morphogenesis in the mammalian embryo remains a formidable challenge. Recently, in vivo models based on embryonic stem cells (ESCs) have emerged as complementary methods to quantitatively dissect the physical and molecular processes that shape the embryo. Here we review recent developments in using embryonic stem cells to create both two and three-dimensio...

several sources were introduced for cell therapy after neurotrauma. there are some evidences that quiescent stem cells are located in the various regions of the adult mammalian brain including cortex, hippocampus, amygdala and striatum. but more study is needed to identify the characteristics of these cells. in the present study we investigated the possibility of isolating neural stem cells fro...

The system-level identification and analysis of molecular networks in mammals can be accelerated by 'next-generation' genetics, defined as genetics that does not require crossing of multiple generations of animals in order to achieve the desired genetic makeup. We have established a highly efficient procedure for producing knock-in (KI) mice within a single generation, by optimizing the genome-...

Organophosphorus chemistry has a crucial role in the field of drug research [1]. As generally well known, Adenosine Triphosphate (ATP) plays key energy processes all living cells mammals. There are also natural phosphorus containing organic compound, Phosphocitrate (PC), found mammalian mitochondria, likely produced by cytosolic phosphorylation citrate, and which important calcium metabolism, e...

In this study we intended to perform in silico and vitro analysis of naturally derived flavonoid, hesperetin as a promising mammalian target rapamycin inhibitor. This features screening natural compound against FK506 binding proteins 12 domain by using Schrodinger glide based virtual further molecular dynamics simulation was done infer the stability complex formation. Mammalian co-crystallized ...

Cervical cancer belongs to the second most frequent kind of in female as well a major cause mortality all over world. MicroRNAs are crucial modulators multiple diseases. Based on GSE86100 database, microRNA-3137 expression was low cervical tissues and difference significant, thus we further probed potential along with mechanism cells. Reverse transcription-quantitative polymerase chain reaction...

Optogenetics uses light as a traceless inducer to remotely control cellular behavior with high safety and spatiotemporal precision, its implementation for therapeutic synthetic biology enable customizable user-defined remedial outputs be generated from suitably engineered cells. Here, we focus on non-neural optogenetics, describing the tools strategies available engineer light-responsive, mamma...