The inhibitory effect on human and rat intestinal disaccharidase by the extractive from the leaves of Morus alba (ELM) containing 0.24 % 1-deoxynojirimycin equivalent and its inhibitory activities were investigated by the modified Dahlqvist method. In the presence of 1000-fold diluted ELM solution, the sucrase activity of four human samples was inhibited by 96 % and that of maltase and isomalta...

In vitroantimicrobial activity, DNA cleavage, anti-diabetic, antioxidant, anti-inflammotary and antihaemolytic of some synthesized Metallocephradines and Metallocefepimes were examined. In a broadspectrum of antibacterial and antifungal property, it was found that nickel (1:1) and copper (1:2) complexes were more active than cephradine against all tested bacteria except S.pyogenes and E.fecalis...

House sparrow nestlings are fed primarily on insects during the first 3 days of their life, and seeds become gradually more important afterwards. We tested whether developmental changes in size and functional capacity of the digestive tract in young house sparrows are genetically hard-wired and independent of diet, or can be modified by food type. Under laboratory conditions, we hand-fed young ...

Background: Cutoff activities for diagnosing disaccharidase deficiencies are historical and are difficult to verify from a reference population. The objectives of this study were to validate the utility of historical disaccharidase cutoffs using data from clinical samples and to evaluate the demographics of individuals for whom intestinal disaccharidase testing was performed. Methods: Results f...

The milk/solid feed intake pattern and digestive capacity were compared in two groups of young rabbits reared between 16 and 32 days in litters of four (L4 group, n = 18 litters) or ten pups (L10, n = 20) in order to modulate milk availability. Compared to the L4 group, rabbits in the L10 group presented a lower milk intake (22.3 versus 28.8 g x day(-1)) and a higher solid feed intake (12.9 ver...

We examined the inhibitory effects of aqueous ethanol extract from mulberry leaves (ME) on postprandial hyperglycemia in normal Wistar rats. ME dose-dependently suppressed the postprandial rise of blood glucose in rats, when ME (0.02-0.5 g/kg) was given 0.5 h before the administration of carbohydrates such as sucrose, maltose and starch. The ME dose showing 50% inhibition of the increment of bl...

Maltase-glucoamylase and sucrase-isomaltase are two human glycosidases responsible for starch digestion. We have performed a comparative analysis of their amino acid sequences from several species of mammals and their orthologues from other chordates. This allowed us to determine the evolutionary history of the enzymes. Both glycosidases are paralogues and contain GH31 family catalytic domains....

This work concerns the degradation of starch to glucose by enzymes of Clostridium acetobutylicum, a stage in the butanol-acetone fermentation which has so far been little investigated. Previous workers have mainly used growing cultures of Cl. acetobutylicum, though Robinson (1922) showed that starch (and other carbohydrates) were fermented by toluene-treated cell suspensions. In the present wor...

Glycogen storage disease type II (GSD II/glycogenosis type II/Pompe's disease/acid maltase deficiency) is caused by the deficiency of lysosomal alpha-glucosidase resulting in lysosomal accumulation of glycogen. The disease is inherited as an autosomal recessive trait and is clinically heterogeneous. Early and late onset phenotypes are distinguished. Insight in the molecular nature of the lysoso...