OBJECTIVES The aim of this study was to investigate the antimicrobial activity of a range of commercially available tea tree oil (TTO) products and to evaluate whether formulation plays a significant part in their antiseptic activity. METHODS The antimicrobial activity of the purchased products and control TTO solutions was assessed against Escherichia coli, Staphylococcus aureus, Salmonella ...

In vitro evaluation of colistin susceptibility is fraught with complications, due in part to the inherent cationic properties of colistin. In addition, no reference method has been defined against which to compare the results of colistin susceptibility testing. This study systematically evaluated the available methods for colistin MIC testing in two phases. In phase I, colistin MICs were determ...

Introduction- There is a growing prevalence of fungal infections among immunocompromised patients, and there also concerning rise in resistance to commonly used antifungal medications. As result, drug susceptibility testing has become an invaluable tool for clinicians, specifically the treatment invasive cases where initial treatments have proven ineffective. Methodology- We conducted analysis ...

Thirteen laboratories collaborated to optimize interlaboratory agreement of results of a broth macrodilution procedure for testing three classes of antifungal drugs against pathogenic yeasts. The activities of amphotericin B, flucytosine, and ketoconazole were tested against 100 coded isolates of Candida albicans, Candida tropicalis, Candida parapsilosis, Candida lusitaniae, Torulopsis (Candida...

A new antibiotic, ascochlorin, C23H29O4CI, was obtained from the filter cake of the fermented broth of Ascochyta viciae Libert. Ascochlorin inhibits plaque formation of both DNA and RNA viruses in the agar-diffusion plaque inhibition method. However, it showed no antiviral activity by the tube culture method. It showed cytotoxicity to chick embryo fibroblast monolayer and HeLa cells at a concen...

Five broth media and two solid media were compared for their ability to quantitatively recover total coliform bacteria from chemically treated water. M-Endo LES and mT7 media were used in the membrane filter technique. Lauryl tryptose broth, lactose broth, presence-absence broth, lactose broth with twice the amount of lactose, and lauryl tryptose broth with twice the amount of sodium lauryl sul...

We examined the effects of temperature and inoculum on the agreement of macro- and microdilution broth MICs of five antifungal agents against six isolates of Candida species or Torulopsis glabrata. Incubation temperature affected results with amphotericin B, flucytosine, fluconazole, and SCH 39304, producing better agreement at 35 degrees C than at 37 degrees C. Agreement between methods was be...