Various organic and aqueous extracts of aerial part of Boenninghausenia albiflora (Rutaceae) obtained by infusion and maceration were screened for their antimicrobial activity against eight animal and plant pathogenic bacteria (Bacillus subtilis, Staphylococcus aureus, Erwinia chrysanthemi, Escherichia coli, Proteus vulgaris, Xanthomonas phaseoli, Agrobacterium tumefaciens and Xanthomonas campe...

Polygalacturonase (PG) was purified from Erwinia carotovora EC. A hybrid cosmid, pSH711, that encodes PG activity but not pectate lyase activity was identified from an E. carotovora genomic library by an immunological screening method. A cell extract of Escherichia coli cells containing pSH711 was able to produce plant tissue maceration when spotted on carrot, potato, or turnip slices. In addit...

Today, natural medicines not only provide the primary health-care needs for themajority of population in developing countries but have attracted more and moreattention developed due to soaring costs universalfinancial austerity. In USA, approximately 49% has tried naturalmedicines prevention treatment diseases [1]. Chemicals known havemedicinal benefits are considered be “active ingredients” or...

This presentation summarizes methods available for killing poultry during outbreaks of epizootic or zoonotic diseases. Available methods for killing poultry are listed and discussed with relation to practical aspects, biosecurity, animal welfare, occupational hazards and other pros and cons of each method. The stunning methods mentioned are blow to the head, electrical stunning and captive bold...

Abstract Soursop ( Annona muricata ) is a plant that widely available in Indonesia. Of all parts of the soursop plant, leaves are most interesting to be studied. part investigated because have many benefits and been applied health sector, both traditional modern. This contain active compounds such as alkaloids, terpenoids, flavonoids, tannins, saponins, acetogenins, others. The purpose this rev...

Ecology of the lactic acid bacteria (LAB) during alcoholic fermentation (AF) and spontaneous malolactic fermentation (MLF) of Tempranillo wines from four wineries of La Rioja has been studied analyzing the influence of the winemaking method, processing conditions, and geographical origin. Five different LAB species were isolated during AF, while, during MLF, only Oenococcus oeni was detected. A...

In this study, we report a modified CTAB-PVP method combined with silicon dioxide (silica) treatment for the extraction of high quality genomic DNA from a single larva or pupa. This method efficiently obtains DNA from small specimens, which is difficult and challenging because of the small amount of starting tissue. Maceration with liquid nitrogen, phenol treatment, and the ethanol precipitatio...

Dickeya dadantii is a broad host range phytopathogenic bacterium provoking soft rot disease on many plants including Arabidopsis. We showed that, after D. dadantii infection, the expression of the Arabidopsis BOS1 gene was specifically induced by the production of the bacterial PelB/C pectinases able to degrade pectin. This prompted us to analyze the interaction between the bos1 mutant and D. d...

The study was carried out to optimize pumpkin oil recovery in the process of aqueous extraction preceded by enzymatic maceration of seeds, as well as to compare the quality of the obtained oil to the quality of cold-pressed pumpkin seed oil. Hydrated pulp of hulless pumpkin seeds was macerated using a 2% (by mass) cocktail of commercial pectinolytic, cellulolytic and proteolytic preparations (R...