the objective of the research was to test the effect of iron and aluminium coagulants commonly used for lake re-cultivation, on the pike sperm motility and fertilisation. the coagulants caused changes in the analyzed hydrochemical parameters of the water such as: fe, mg2+, tss, so42- and cl-. selected parameters of sperm motility: mot, vcl, vsl, vap, lin were estimated using casa. the sperm mot...

In this work, we report a new method to reversibly immobilize proteins to a surface in a functionally active orientation directly from cell lysate by employing a fusion protein consisting of a thermal-responsive elastin (ELP) domain as the surface anchor and a calcium-responsive calmodulin (CalM) domain for protein capturing. Incorporation of an M13 tag into recombinant proteins enables not onl...

PPARs are nuclear receptors activated by ligands. Activation of PPARγ leads to a reduction of adhesion and motility in some cancer models. PPARγ transcriptional activity can be negatively regulated by JNK-mediated phosphorylation. We postulated that the use of agents able to inhibit JNK activity could increase the effectiveness of PPARγ ligands. We analysed the effects of rosiglitazone (PPARγ l...

In a previous study (Shin, E. Y., Shin, K. S., Lee, C. S., Woo, K. N., Quan, S. H., Soung, N. K., Kim, Y. G., Cha, C. I., Kim, S. R., Park, D., Bokoch, G. M., and Kim, E. G. (2002) J. Biol. Chem. 277, 44417-44430) we reported that phosphorylation of p85 betaPIX, a guanine nucleotide exchange factor (GEF) for Rac1/Cdc42, is a signal for translocation of the PIX complex to neuronal growth cones a...

BACKGROUND INFORMATION Cell motility entails the reorganization of the cytoskeleton and membrane trafficking for effective protrusion. The GIT-PIX protein complexes are involved in the regulation of cell motility and adhesion and in the endocytic traffic of members of the family of G-protein-coupled receptors. We have investigated the function of the endogenous GIT complexes in the regulation o...

This work briefly discusses the potential applications of Skipper-CCD technology in astronomy and reviews its current use dark matter neutrino experiments. An overview ongoing efforts to build multi-kilogram experiments with these sensors is given, context Oscura experiment. First results from characterization first 200 mm wafer-fabrication run a new vendor are presented. The overall yield elec...

The major coat protein (gene 8 product) of coliphage M13 is an integral protein of the host cell cytoplasmic membrane prior to virus assembly. It is synthesized as a precursor, termed procoat, with an extra 23 NH2terminal residues. We have studied the synthesis, assembly, and processing of procoat protein by amino acid pulse-labeling E. coli which are infected by either M13 or by M13 with amber...

a Neuroscience & Psychiatry Unit, Stopford Building, The University of Manchester, Oxford Road, Manchester M13 9PL, UK b Centre for Biostatistics, Jean McFarlane Building, The University of Manchester, Oxford Road, Manchester M13 9PL, UK c Brain Mapping Unit, Herchel Smith Building for Brain and Mind Sciences, University of Cambridge, Robinson Way, Cambridge CB2 0SZ, UK d Imaging Sciences, Stop...