A lytic bacteriophage RG-2014 infecting a biofilm forming multidrug resistant bacterium Delftia tsuruhatensis strain ARB-1 as its host was isolated from a full-scale municipal wastewater treatment plant. Lytic phage RG-2014 was isolated for developing phage based therapeutic approaches against Delftia tsuruhatensis strain ARB-1. The strain ARB-1 belongs to the Comamonadaceae family of the Betap...

A naturally occurring Salmonella mikawasima serologically converted by phage 14 (6,7,14:y:e,n,z15) has been isolated for the first time. An S. tennessee variant seroconverted by phage 14 (6,7,14:z29:-) was also isolated. The source of these salmonellae was the common freshwater aquarium snail Ampullaria. Phage 14 prepared from these serovariants was lytic for S. bovis-morbificans (6,8:r:1,5) an...

Salmonella enterica serovar Enteritidis has remained a major food-borne pathogen in humans. We isolated a virulent S. enterica serovar Enteritidis bacteriophage, SE2, which belongs to the family Siphoviridae. Phage SE2 could lyse S. enterica serovar Enteritidis PT-4, and its virulence was maintained even at ambient temperature. The genomic sequence of phage SE2 was composed of 43,221 bp with cl...

A novel Pseudomonas aeruginosa lytic bacteriophage (phage), PA1Ø, was isolated, and its genome was sequenced completely. This phage is able to lyse not only P. aeruginosa but also Staphylococcus aureus. Genome analysis of PA1Ø showed that it is similar to a P. aeruginosa temperate phage, D3112, with the exception of the absence of a c repressor-encoding gene, which is known to play a critical r...

90 Virulence Volume 4 Issue 1 Phage lytic enzymes have potential as new inroads toward novel antibiotics. Until now this approach has only been promising for Grampositive bacteria because in Gram-negatives the target of lytic action is protected by an outer envelope. Information gleaned from the structural studies of two plague proteins— pesticin and FyuA—allowed us to engineer a “hybrid” prote...

Outnumbering all other biological entities on earth, bacteriophages (phages) play critical roles in structuring microbial communities through bacterial infection and subsequent lysis, as well as through horizontal gene transfer. While numerous studies have examined the effects of phages on free-living bacterial cells, much less is known regarding the role of phage infection in host-associated b...

16 Phage-coded lysin is an enzyme that destroys the cell wall of bacteria. Phage lysin 17 could be an alternative to conventional antibiotic therapy against pathogens that are 18 resistant to multiple antibiotics. In this report, a novel staphylococcal phage, GH15, was 19 isolated and the endogenous lytic enzyme (LysGH15) was expressed and purified. The 20 lysin LysGH15 displayed a broad lytic ...

The lytic-lysogenic switch of the temperate lactococcal phage TP901-1 is fundamentally different from that of phage lambda. In phage TP901-1, the lytic promoter PL is repressed by CI whereas repression of the lysogenic promoter PR requires the presence of both of the antagonistic regulator proteins, MOR and CI. We model the central part of the switch and compare the two cases for PR repression:...

TM4 is a lytic mycobacteriophage which infects mycobacteria of clinical importance. A luciferase reporter phage, phAE40, has been constructed from TM4 and was previously shown to be useful for the rapid detection and drug susceptibility testing of Mycobacterium tuberculosis. However, the lytic nature of the phage results in a loss of detectable light output and limits the sensitivity of detecti...

We report the complete genome sequences of two Edwardsiella tarda-lytic bacteriophages isolated from flounder kidney (KF-1) and seawater (IW-1). These newly sequenced phage genomes provide a novel resource for future studies on phage-host interaction mechanisms and various applications of the phages for control of edwardsiellosis in aquaculture.