Electrostimulation (ES) can be defined as a safe physical method to induce stem cell differentiation. The aim of this study is to evaluate the effectiveness of ES on bone marrow mesenchymal stem cells (BMSCs) seeded in collagen scaffolds in terms of proliferation and differentiation into cardiomyocytes. BMSCs were isolated from Wistar rats and seeded into 3D collagen type 1 templates measuring ...

Gerald Gentleman Station (GGS), located in southwestern Nebraska near Sutherland, is the newest and largest electric generating facility in the Nebraska Public Power District (NPPD) system. In August 1994, NPPD constructed a 9.2-mile long railspur between GGS and the Union Pacific Railroad to create competition in the coal-hauling market. A multi-objective criteria analysis was used to identify...

There is a need to develop three-dimensional structures that mimic the natural islet tissue microenvironment. Endocrine micro-pancreata (EMPs) made up of acellular organ-derived micro-scaffolds seeded with human islets have been shown to express high levels of key beta-cell specific genes and secrete quantities of insulin per cell similar to freshly isolated human islets in a glucose-regulated ...

The use of covers may improve establishment seeded turfgrass but their in combination with drip irrigation techniques has not been evaluated. We investigated the effects two cover types and three methods on tall fescue turfgrass. For spring seeding [Schedonorus arundinaceus (Schreb.) Dumort.], turf was successful subsurface (SDI) aboveground (AGD) fine textured soil transition zone. With SDI, A...

The impact of low-intensity diffuse ultrasound (LIDUS) stimulation on the cytoskeletal organization of chondrocytes seeded in three-dimensional (3D) scaffolds was evaluated. Chondrocytes seeded on 3D chitosan matrices were exposed to LIDUS at 5.0 MHz (approx. 15 kPa, 51 s, 4 applications/day) in order to study the organization of actin, tubulin and vimentin. The results showed that actin presen...

Bearing failure is often attributed to be one of the major causes of breakdown in industrial rotating machines that operate at high and low speeds. This paper presents results of a comparative experimental study on the application of the ultrasound technique for condition monitoring of low speed rolling element bearings and conventional vibration measurements with seeded faults on inner-race de...

To investigate a possible correlation between cytodifferentiation, proliferation, and actin expression, smooth muscle cells from the 9-week-old rabbit aortic media were enzyme-dispersed into single cells and were plated in primary culture at different initial seeding densities. The volume fraction of myofilaments (Vv myo) in cells seeded moderately densely fell from 39.5% +/- 1.2% in the intact...

This study describes a novel fabrication technique to grow gold nanoparticles (AuNPs) directly on seeded ZnO sacrificial template/polymethylsilsesquioxanes (PMSSQ)/Si using low-temperature hydrothermal reaction at 80°C for 4 h. The effect of non-annealing and various annealing temperatures, 200°C, 300°C, and 400°C, of the ZnO-seeded template on AuNP size and distribution was systematically stud...