Title: Activation state of the hyperpolarization-activated current (Ih) modulates temperature-sensitivity of firing in locus coeruleus neurons from bullfrogs Author Names: Joseph M. Santin & Lynn K. Hartzler Author Contributions: J.S. & L.H. conception and design of research; J.S. performed experiments; J.S. analyzed data; J.S. & L.H. interpreted results; J.S. wrote the manuscript; J.S. & L.K. ...

4 Ningren Cui*, Xiaoli Zhang*, Jyothirmayee S. Tadepalli, Lei Yu, Hongyu Gai, James Petit, 5 Ravi T. Pamulapati, Xin Jin and Chun Jiang 6 7 Department of Biology, Georgia State University, 100 Piedmont Avenue, Suite 495 8 P.O. Box 4010, Atlanta, GA 30302 9 10 11 12 13 Running head: TRP channels in neuronal CO2 chemosensitivity 14 15 Number of text pages: 28 16 17 Number of display items: 10 18 ...

It has been proposed that the locus coeruleus (LC) regulates nonspecific arousal and thereby may participate in a wide range of functions. Our work indicates that, while the LC may indeed play an important role in arousal, it has more specific effects on behavior and may regulate cortical mechanisms involved in selective attention and task performance. In one study, we recorded impulse activity...

The purpose of this study was to investigate the ionic and second messenger mechanisms underlying the hyperpolarizations induced by the selective alpha 2-adrenoceptor agonist clonidine and the opiate agonist morphine in the locus ceruleus. Intracellular recordings were carried out in rat brain slices, and drugs at known concentrations were administered in the perfusate. The cyclic adenosine 3':...

The μ-opioid receptor is the primary site for the action of morphine. In the present study, we investigated the regulation of the μ-opioid receptor mRNA levels in the locus ceruleus, ventral tegmental area, nucleus accumbens and hypothalamus of the rat brain following intracerebroventricular administration of morphine for 7 days. The isolated mRNA from these regions was subjected to real-time q...

Metabotropic transmission typically occurs through the spillover activation of extrasynaptic receptors. This study examined the mechanisms underlying somatodendritic dopamine and noradrenaline transmission and found that the extent of spillover and pooling varied dramatically between these two transmitters. In the mouse ventral tegmental area, the time course of D2-receptor-mediated IPSCs (D2-I...

Increased sympathetic outflow from the central nervous system to the periphery may contribute to the initiation of hypertension in spontaneously hypertensive rats (SHR). As this alteration in sympathetic activity may be mediated in part by a-adrenergic receptors in the central nervous system, the current study examined aradrenergic receptors in various brain areas of SHR and normotensive Wistar...

To investigate the release profile of met-enkephalin, β-endorphin, and dynorphin-A in ruminants' CNS, goats were stimulated by electroacupuncture of 0, 2, 40, 60, 80, or 100 Hz for 30 min. The pain threshold was measured using potassium iontophoresis. The peptide levels were determined with SABC immunohistochemisty. The results showed that 60 Hz increased pain threshold by 91%; its increasing r...

Unilateral injection of 6-hydroxydopamine into the locus ceruleus led to long lasting depletion of norepinephrine in the ipsilateral parietal cerebral cortex of the rat up to 8 weeks after injection. The uptake of both norepinephrine and dopamine by homogenates of parietal cortex was decreased markedly on the ipsilateral, but not on the contralateral, side. The uptake of four other neurotransmi...