We studied cavitation in metastable fluids drawing on the example of liquid nitrogen confined to spheroidal pores of specially prepared well-characterized mesoporous silica materials with mean pore diameters ranging from approximately 6 to approximately 35 nm. Cavitation was monitored in the process of evaporation/desorption from fully saturated samples with gradually decreasing vapor pressure ...

Suspensions of Lactobacillus leichmannii were stored in liquid nitrogen and were used as direct inocula in vitamin B(12) assays. Complete recovery of viable cells was obtained when the suspensions in basal B(12) medium were rapidly frozen by direct immersion into liquid nitrogen and rapidly thawed by agitating the suspensions in a water bath at 40 C. Greater than 90% destruction occurred when t...

OBJECTIVE To determine the efficacy of combination treatment of Albendazole along with liquid nitrogen in cutaneous larva migrans. STUDY DESIGN Quasi-experimental study. PLACE AND DURATION OF STUDY Abbasi Shaheed Hospital and The Aga Khan Hospital, Karachi, from December 2008 to December 2010. METHODOLOGY Eighteen cases of cutaneous larva migrans were collected and divided into two groups...

An immortalized mouse fetal liver stromal cell line, named KM3, has demonstrated the potential to support the growth and maintenance of human embryonic stem cells (hESCs). In this study, the characteristics of KM3 cells were examined following cryopreservation at -70°C and in liquid nitrogen for 15, 30 and 60 days following treatment with 10 μg/ml mitomycin C. In addition, whether the KM3 cells...

The objectives of this study were to compare the efficiency of three methods for repairing tarsal defects (glycerine-preserved, alcohol-preserved and cryopreserved tarsal plate and palpebral conjunctival transplantation) based on histopathological changes and apoptosis, and to evaluate the clinical effects of repairing tarsal defects by liquid nitrogen-cryopreserved tarsal plate and palpebral c...

The larva of the drosophilid fly, Chymomyza costata, is probably the most complex metazoan organism that can survive submergence in liquid nitrogen (-196 °C) in a fully hydrated state. We examined the associations between the physiological and biochemical parameters of differently acclimated larvae and their freeze tolerance. Entering diapause is an essential and sufficient prerequisite for att...

OBJECTIVE To evaluate cryodamage on human sperm motility, cryosurvival rate, and sperm chromatin assessed by acridine orange staining method (AO test) after a six-month freeze-thawing process using liquid nitrogen vapor. STUDY DESIGN Experimental study. MATERIAL AND METHOD Twenty normal semen samples were obtained from the male partner of infertile couples attending the infertility unit, Si...

PURPOSE To report a case series of conjunctival lymphangiectasia treated with liquid nitrogen cryotherapy. METHODS A 1.5-mm Brymill cryoprobe was applied in a double freeze-thaw method after an incisional biopsy of a portion of the conjunctiva in patients with conjunctival lymphangiectasia. Freeze times were 1 to 2 seconds with thawing of 5 to 10 seconds between treatments. Patients were reex...

1. A wide variety of procedures was used to test the motility of mammalian sperm after plunging them into liquid nitrogen at -195 degrees C. and later rapidly warming them to 35 degrees C. by plunging them into a suitable balanced and isotonic medium. 2. Using seminal fluid sperm from the same human donor, maximal numbers of motile sperm survived vitrification when the samples were (a) very fre...

The effects of vitrification and cryostorage length in liquid nitrogen (LN2) on in vitro survival of rabbit embryos were examined. A total of 374 morphological normal embryos collected from 20 superovulated multiparous New Zealand White rabbit were divided into two groups. The first group (n=94) was non-vitrified used as a fresh control group and the second group (n=280) was vitrified and stora...