To evaluate the effect of expression of latent membrane protein (LMP) 1 encoded by Epstein-Barr virus (EBV) on Cepsilon mRNA expression, mRNA levels were examined by RT-PCR or Northern blot analysis upon transient transfection of LMP1 in the splenocytes derived from Brown-Norway rats with or without immunization with 2,4-dinitrophenyl-conjugated Ascaris suum antigen. Splenocytes were transfecte...

• In vitro , lipofection of CCL5/CCL17 modifies the expression cytokines. The main driver these changes was entry foreign DNA into cytosol. vivo ECT pulses lead to high Ccl5 and Ccl17 after GET. GET CCL5 or CCL17 results in increased Cxcl9 Ifn? . are potent activators immune system CT26 tumor model. effectiveness immunotherapy highly correlates with degree type infiltrated cells tissue. Treatme...

Sperm mediated gene transfer can be an inexpensive and simple method in animal transgenesis; however its efficiency is poor, mainly due to the spermatozoa’s lesser uptake of exogenous DNA. In the present study, the effects of lipofection and other augmentation techniques, such as sperm freezing and spermatozoa treatment with triton X100 and DMSO, on exogenous DNA uptake by sheep spermatozoa and...