Acinetobacter sp. isolated from healthy human skin of a tribal population was tested for lipase production.Medium optimization was achieved to increase the production. Purification was carried out by a one-step purification process using DEAE Sephadex A-50. The molecular weight of the lipase was approximately 60 kDa by SDS-PAGE. The purified lipase showed not only good stability in the presence...

We compared two methods for the direct selective measurement of hepatic lipase and lipoprotein lipase activities in human plasma after intravenous administration of heparin. Except for the emulsifier (gum arabic vs lecithin), the two assay media for hepatic lipase are essentially similar. Results for hepatic lipase by these two assays correlate well (r = 0.99). The assays for lipoprotein lipase...

Human monocyte-derived macrophages in culture produced lipoprotein lipase. Although freshly isolated blood monocytes did not secrete much lipase activity, 1 d in culture was sufficient to trigger measureable enzyme production. During 3 wk in culture, maximal activity was attained after 7 d. At all times, the culture medium contained more enzyme activity than did a serum-heparin eluate or a dete...

Several workers (l-3) have reported that lipase activity assayed in homogenates of adipose tissue is increased by exposure of the tissue to epinephrine or to adrenocorticotropic hormone prior to homogenization. In general, the magnitude of the increases observed has been small compared to the large effects of these hormones on glycerol format,ion by the intact tissae. In studies of whole homoge...

The development of lipoprotein lipase has been examined during adipose conversion of preadipocyte Ob17 cells. These cells have been previously shown to differentiate in clusters of fat cells. The lipoprotein lipase activity, which increases 20-50-fold during adipose conversion, is fully inhibited by anti-lipoprotein lipase y-globulins (directed against the rat heart enzyme) and is heparin-relea...

BACKGROUND We present the case of an 80-year-old woman who was admitted to hospital with an intermittent volvulus of the right colon. A total colectomy was performed. Initially, serum amylase and lipase increased concordantly, but after a few weeks amylase normalized (85 U/L), whereas lipase increased to 3764 U/L. This discrepancy and persistence of hyperlipasemia suggested a macromolecular for...

The study is attempted to investigate the production of potent lipase from groundnut oil mill fungi. The fungi was isolated and identified as Trichoderma sp. Lipolytic activity of Trichoderma sp. was preliminarily confirmed by Rhodamine B method, lipase production was carried out using mineral medium. The extracellular protein concentration and lipase enzyme activity (7.83U/ml) was maximum at 4...

Patients with cystic fibrosis have been found to have abnormal serum concentrations of immunoreactive trypsin and abnormal activities of pancreatic isoamylase. A study was undertaken to discover whether activity of pancreatic lipase is also altered in cystic fibrosis. Serum from 23 patients with cystic fibrosis was assayed for immunoreactive trypsin and pancreatic lipase. Median serum pancreati...

The esterification activity of microbial lipase (triacyl glycerol hydrolase, E.C. 3.1.1.3) in water-organic biphasic solvent system has been investigated. The lipase produced from Rhizopus arrhizus NCM997 strain by solid-state fermentation, using rice bran as substrate, exhibited novel capability of catalyzing esterification reaction. The partially purified lipase was coated with nonionic surfa...

Kulkarni BV, Mattes RD. Lingual lipase activity in the orosensory detection of fat by humans. Am J Physiol Regul Integr Comp Physiol 306: R879–R885, 2014. First published April 2, 2014; doi:10.1152/ajpregu.00352.2013.—Lingual lipase generates nonesterified fatty acids (NEFA) from dietary fats during oral processing by lipolysis. Lingual lipase in rodents has strong lipolytic activity and plays ...