Bovine viral diarrhea virus (BVDV) is an economically important pathogen of cattle that is maintained in the population by persistently infected animals. Virus infection may result in reproductive failure, respiratory disease and diarrhoea in naïve, susceptible bovines. Here, the construction and characterization of a novel vectored vaccine, which is based on the incorporation of genes encoding...

Addressing the envelope for gene delivery ---------------------------------------------------------------------------------------------------------------Richard J. O’Reilly1 1MEMORIAL SLOAN-KETTERING CANCER CENTER In this issue of Blood, Zhou et al describe a strategy for modifying the envelope proteins of a lentiviral vector to display a single chain antibody fragment, or ScFv, specific for CD...

bovine viral diarrhea virus (bvdv) is a pestivirus of the flaviviviridaefamily that infects cattle, and its widespread distribution can cause substantial economic losses worldwide. the aims of this study were: (1) to estimate the prevalence of animals persistently infected (pi) with bvdv in industrial dairy cattle herds in suburb of mashhad- iran; and (2) to compare the occurrence of persistent...

Hematopoietic stem cell gene therapy requires the use of integrating retroviral vectors in order to stably transmit a therapeutic gene to mature blood cells. Human clinical trials have shown that some vector integration events lead to disrupted regulation of proto-oncogenes resulting in disordered hematopoiesis including T-cell leukemia. Newer vectors have been designed to decrease the incidenc...

The delivery of adipose-derived stem cells (ADSCs) for promoting tissue repair has become a potential new therapy, while hepatocyte growth factor (HGF) is an important growth factor with angiogenic, anti-fibrotic, and anti-inflammatory benefits. In this paper, hADSCs were separated, cultured and identified based on the expression of cell surface antigens and multiple differentiation potential. ...

Mucopolysaccharidosis type I (MPS I) is a lysosomal disease caused by α-Liduronidase (IDUA) deficiency and subsequent accumulation of glycosaminoglycan (GAG) heparan sulfate and dermatan sulfate. Lentiviral vector encoding correct IDUA sequence could be used for gene therapy treating MPS I disease. Previous study in our lab showed that intravenous injection of a lentiviral vector (CSP1) into ne...

BACKGROUND/AIMS Stem cell transplantation and gene therapies have been shown to attenuate myocardial dysfunction after myocardial infarction (AMI) in different acute and chronic animal models. The aim of this study was to assess the potential therapeutic efficacy of endothelial NO synthases (eNOS)-expressing endothelial progenitor cells (EPCs) on infarcted hearts. METHODS Lentiviral eNOS-infe...

The aim of the present study was to investigate the feasibility of lentiviral‑mediated Cys‑Asn‑Gly‑Arg‑Cys (CNGRC) peptide gene transduction in adipose stem cells. Adipose stem cells were prepared using enzymatic digestion and repeated adherence methods and identified in culture by immunofluorescence staining of surface markers. The pluripotency of the cultured adipose stem cells was confirmed ...