On the basis of partial amplification of a cloned fragment of kDNA of Leishmania infantum which is specific for this species, we developed a PCR-ELISA technique which avoids the problems associated with classical diagnostic techniques. This technique was tested on 33 L. infantum strains from 19 different zymodemes, which were recognized equally. It was also used on human and canine clinical sam...

A cross-sectional serosurvey using Leishmania infantum ELISA was performed on 445 cats living in ecoregions around the Northwestern Mediterranean basin; 58 cats from an area of the US where leishmaniasis is not endemic were used as negative controls. ELISA results were further confirmed in 69 cats by Western blot (WB). Finally, 76 of them were also tested for FeLV and FIV. Seroprevalence by ELI...

OBJECTIVES To evaluate the in vitro leishmanicidal activity of nine flavonoid derivatives from Delphinium staphisagria against L. infantum and L. braziliensis. DESIGN AND METHODS The in vitro activity of compounds 1-9 was assayed on extracellular promastigote and axenic amastigote forms and on intracellular amastigote forms of the parasites. Infectivity and cytotoxicity tests were carried on ...

The serologic assay is an important tool in the diagnosis of leishmaniasis. One of the most commonly used tests is enzyme-linked immunosorbent assay (ELISA). Since total Leishmania promastigotes are used as antigen in the routine assay, false-positive reactions are frequent due to cross-reaction with sera from other diseases, mainly Chagas' disease. Therefore, an antigen that determines less cr...

conclusions artemether especially in oral treatment is an effective and simple method and may be used as a new method to treat visceral leishmaniasis. results ic50 of the drug on leishmania infantum was determined to be 25 μg/ml after 24 h. in vivo experiments indicated that oral artemether treatment of mice, during 3 days and every 6 h (0.625 mg/kg) was more significant than parenteral (0.625 ...

Citation: Lázaro-Souza M, Matte C, Lima JB, Arango Duque G, Quintela-Carvalho G, de Carvalho Vivarini Á, Moura-Pontes S, Figueira CP, Jesus-Santos FH, Gazos Lopes U, Farias LP, Araújo-Santos T, Descoteaux A and Borges VM (2018) Leishmania infantum Lipophosphoglycan-Deficient Mutants: A Tool to Study Host Cell-Parasite Interplay. Front. Microbiol. 9:626. doi: 10.3389/fmicb.2018.00626 Leishmania ...

Background: Ehrlichia canis and Babesia vogeli comprise a group of globally distributed pathogens transmitted by ticks. Leishmania infantum is transmitted by Lutzomyia longipalpis, etiological agent of leishmaniasis. The pathogens affect the animals; and can also affect the human. An imbalance between oxidants and antioxidants compounds causes an increase in free radicals, and other reactive ox...

Vector-borne diseases (VBD) are caused by a range of pathogens transmitted by arthropods and have emerged in recent years, showing a wider geographic distribution and increased global prevalence. In addition to their veterinary medical importance, cats play a central role in the transmission cycles of some VBD agents by acting as reservoirs, amplifying hosts or sentinels. The aim of this study ...