microglia cells are a subset of central nervous system (cns) macrophages. changes in the cns such as injury, or developmental events, follow by morphological and physiological changes in microglia cells. in this study organotypic brain slice cultures under serum free condition were used to investigate the morphology and lectin histochemistry of microglia and macrophages in the cns in vitro. mic...

background: the aim of this research was to study the distribution and changes of glycoconjugates particularly their terminal sugars by using lectin histochemistry during mouse spinal cord development. methods: formalin-fixed sections of mouse embryo (10-16 fetal days) were processed for lectin histochemical method. in this study, two groups of horseradish peroxidase-labeled specific lectins we...

An 8-year-old male Tibetan Terrier showed prolonged astasia, complete paralysis, ticlike signs, and seizure and died 2 months after the onset of symptoms. Histopathologically, there was moderate to severe infiltration of pleomorphic histiocytic mononuclear cells bilaterally in the basiarachnoidal and ventricular areas of the brain. The spinal dura mater, arachnoidal space, and leptomeninges wer...

BACKGROUND Identification of cell types in bovine tissue sections is complicated by the limited availability of anti-bovine antibodies, and by antigen retrieval treatments required for formalin-fixed tissue samples. We have evaluated an antibody and lectin panel for identifying major cell types in paraffin-embedded bovine tissue sections, and report optimized pretreatments for these markers. ...

In the present research we have studied the distribution and the changes of the oligosaccharidic component of the glycoconjugates during the development of the choroids plexuses of the 3rd ventricle and of the lateral ventricles in chick embryos and in 3-day-old chicken. For this purpose a battery of seven horseradish peroxidase labelled lectins was used. Lectin histochemistry has shown that st...

The expression of cell surface oligosaccharides is associated with various biological phenomena. To clarify the relationship between lectin binding and the survival of patients with Burkitt's lymphoma, tumor samples from nine patients with Burkitt's lymphoma were analyzed by lectin histochemistry. Kaplan-Meier analysis showed that survival is significantly shorter for patients with negative rea...

The stomach of three species of non-human primates was investigated by lectin histochemistry to clarify the staining affinity and distribution patterns of their sugar residues. All gastric regions, with little differences between the deep and superficial parts of the same region, were rich in. in N-acetylglucosamine and/or neuraminic acid. Although, the superficial regions of the gastric mucosa...

introduction: the cell surface glycoconjugate and extracellular matrix may mediate extracellular signals which decide the fate of the cells during tissue interactions. the proper development of cornea needs cellular interaction between surface epithelium, neural crest and developing lens. materials and methods: 50 pregnant rats were chosen and the embryos from pernatal day11-to 20 and postnatal...

conclusions our histochemical findings showed a relatively heavy glycosylation of syncytiotrophoblasts of both partial and complete molar tissues, which was prominent in apical portion. this may play a role in their capacity to increase trophoblastic proliferation. results the partial and complete moles generally showed similar reactivity with all used lectins. none of lectins reacted with vill...